Recombinant protein having bacteriophage endosialidase enzymatic activity
AssigneeEndozyme Limited
InventorsPeter William Taylor, John Paul Luzio, Jonathan Mark Bryant
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Abstract
A recombinant protein having bacteriophage endosialidase enzymatic activity obtainable by expression from a recombinant vector comprising a DNA sequence encoding a bacteriophage endosialidase linked to a DNA sequence of an expression vector which express a polypeptide which adds to the N-terminus of the endosialidase, or an analogue of said protein, which is a mutant, functional fragment or derivative of said protein having endosialidase enzymatic activity.
Claims18
An isolated and purified polynucleotide consisting of nucleotides 172 to 1744 of SEQ ID NO:1.
A recombinant fusion protein obtained by expression of a recombinant expression vector comprising nucleotides 172 to 1744 of SEQ ID No:1, encoding a bacteriophage endosialidase and linked to a polynucleotide sequence that can express, a polypeptide such that said polypeptide is added to the N-terminus of said bacteriophage endosialidase either directly or through a spacer sequence.
A recombinant fusion protein obtained by expression of a recombinant expression vector comprising nucleotides 1-2436 of SEQ ID NO:1, encoding it bacteriophage endosialidase and linked to a polynucleotide sequence that can express a polypeptide such that said polypeptide is added to the N-terminus of said bacteriophage endosialidase either directly or through a spacer sequence.
A recombinant expression vector comprising nucleotides 172 to 1744 of SEQ ID NO:1, encoding a bacteriophage endosialidase and linked to a polynucleotide sequence that can express a polypeptide such that said polypeptide is added to the N-terminus of said bacteriophage endosialidase wherein said recombinant expression vector is capable of directing expression of a recombinant fusion protein having bacteriophage endosialidase enzyme activity in a compatible host cell.
A process for the production of a recombinant fusion protein having bacteriophage endosialidase enzyme activity, said process comprising:
- culturing a host cell transformed with a recombinant expression vector comprising nucleotides 172 to 1744 of SEQ ID NO:1, encoding a bacteriophage endosialidase and linked to a polynucleotide sequence that can express a polypeptide such that said polypeptide is added to the N-terminus of said bacteriophage endosialidase wherein said recombinant expression vector is capable of directing expression of a recombinant fusion protein having bacteriophage endosialidase enzyme activity in a compatible host cell, under conditions allowing expression of said recombinant fusion protein;
- and
- isolating the recombinant fusion protein thereby produced.
Dependencies are inferred from the claim text.