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OwnerC. Ron Wilson, David L. Craft +8inventors
InventorsC. Ron Wilson, David L. Craft, L. Dudley Eirich, Mark Eshoo, Krishna M. Madduri, Cathy A. Cornett, Alfred A. Brenner, Maria Tang, John C. Loper, Martin Gleeson
Estimating the term…
Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ω-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.
Isolated nucleic acid encoding a CYP52A2A protein having the amino acid sequence set forth in SEQ ID NO:
Isolated nucleic acid comprising a coding region defined by nucleotides 1199-2767 as set forth in SEQ ID NO:
A vector comprising a nucleotide sequence encoding CYP52A2A protein including an amino acid sequence as set forth in SEQ ID NO:
A method of producing a CYP52A2A protein including an amino acid sequence as set forth in SEQ ID NO:
A method for increasing production of a dicarboxylic acid comprising:
A method for increasing the production of a CYP52A2A protein having an amino acid sequence as set forth in SEQ ID NO:
Dependencies are inferred from the claim text.