Method of intracellular binding target molecules
AssigneeDana-Farber Cancer Institute, Inc.
InventorsWayne A. Marasco, William A. Haseltine
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Abstract
The present invention relates to a method by which one can target an undesired target molecule or target antigen, preferably a protein. The method comprises the intracellular expression of an antibody capable of binding to the target. A DNA sequence is delivered to a cell, the DNA sequence contains a sufficient number of nucleotides coding for the portion of an antibody capable of binding to the target operably linked to a promoter that will permit expression of the antibody in the cell(s) of interest. The antibody is then expressed intracellularly and binds to the target, thereby disrupting the target from its normal actions.
Claims22
A method for the intracellular binding of a target antigen which comprises:
- (a) intracellular delivery of an antibody, wherein said antibody either has no secretory sequence or if said antibody has a secretory sequence, said antibody also contains an intracellular localization sequence, and said antibody is functional intracellularly, wherein said function is determined by the ability of the antibody to bind to the target antigen;
- and
- (b) intracellular binding of the target antigen by said antibody.
Dependencies are inferred from the claim text.