Bacillus thuringiensis CryET33 and CryET34 compositions and uses therefor
AssigneeMonsanto Technology LLC
InventorsWilliam P. Donovan, Judith C. Donovan, Annette C. Slaney
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Abstract
Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode respectively the coleopteran-toxic proteins, CryET33 (29-kDa) crystal protein, and CryET34 (14-kDa) crystal protein. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
Claims10
A composition comprising an insecticidally effective amount of a first polypeptide and a second polypeptide, wherein said first polypeptide comprises an amino acid sequence at least 80% identical to SEQ ID NO:4, and said second polypeptide comprises an amino acid sequence at least 80% identical to SEQ ID NO:3;
- and an agriculturally acceptable carrier, wherein the composition is substantially free of intact cells.
Dependencies are inferred from the claim text.