Detection of copy number changes in colon cancer
AssigneeThe Regents of the University of California
InventorsGiovanni E. Pauletti, Dennis J. Slamon
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Abstract
The present invention provides methods of screening for colon carcinoma cells in a sample. The methods comprise providing a nucleic acid sample from a premalignant lesion in colorectal tissue from a human patient and contacting the sample with a nucleic acid probe that selectively hybridizes to a chromosomal region at 20q.
Claims25
A method of screening for colon carcinoma precursor cells in a sample by determining the presence of increased copy number of chromosome 20q, the method comprising:
- providing a nucleic acid sample from a premalignant lesion in colorectal tissue from a human patient;
- contacting the sample with a nucleic acid probe that selectively hybridizes to a chromosomal region on 20q;
- and
- detecting the formation of a hybridization complex, whereby the presence of increased copy number on 20q indicates the presence of colon cancer precursor cells.
A method of screening for colon carcinoma precursor cells in a sample by determining the presence of increased copy number of chromosome 20q13.2, the method comprising:
- providing a nucleic acid sample comprising a metaphase spread or an interphase nucleus from a premalignant lesion in colorectal tissue from a human patient;
- contacting the sample with a fluorescently labeled nucleic acid probe that selectively hybridizes to a chromosomal region at 20q13.2 and
- detecting the formation of a hybridization complex, whereby the presence of increased copy number at 20q13.2 indicates the presence of colon carcinoma precursor cells.
Dependencies are inferred from the claim text.