Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
AssigneeCalipher Technologies Corp.
InventorsHua Yang, Steven A. Sundberg
Life of this patent
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Abstract
Hydrophilic protein adsorption resistant coatings for microfluidic devices are provided. Additionally, microfluidic devices and methods of manufacturing microfluidic devices that include the coatings are provided.
Claims28
A protein adsorption resistant surface having the formula:
- R1—{(R2)a—(R3)m}n
- wherein,
- R1 is a member selected from organic polymers, glass and silica-based polymers;
- R2 is selected from amino acids and peptides;
- R3 is a hydrophilic polymer;
- a is greater than 0;
- m is at least 1;
- and
- n is ≧1.
A protein adsorption resistant surface having the formula:
- R1—{(R2)a—(R3)m}n
- wherein
- R1 is a member selected from organic polymers, glass and silica-based polymers;
- R2 is a member selected from the group consisting of arginine, lysine, aspartic acid, glutamic acid, peptides comprising arginine, lysine, aspartic acid, glutamic acid and combinations thereof;
- R3 is a hydrophilic polymer;
- a is greater than 0;
- m is at least 1;
- and
- n is ≧1.
A protein adsorption resistant surface having the formula:
- R1—R4—{(R2)a—(R3)m}n
- wherein,
- R1 is a member selected from organic polymers, glass and silica-based polymers;
- R2 is selected from amino acids and peptides;
- R3 is a hydrophilic polymer;
- R4 is a group having the formula
- X—R5—Y;
- in which:
- R5 is —(CH2)s-;
- X and Y are the same or different and are inert linking groups;
- and
- s is at least 3;
- a is greater than 0;
- m is at least 1;
- and
- n is ≧1.
A protein adsorption resistant surface having the formula:
- R1—{(R2)a—(R3)m}n
- wherein,
- R1 is a member selected from organic polymers, glass and silica-based polymers;
- m is 1 and —R2—R3 has the formula
- in which:
- each R6 is independently selected from
- H, —CH3, —CH2)3NHC(NH)NH2;
- —CH2CO2H;
- —(CH2)2CO2H or —(CH2)4NH2;
- R7 is H or CH3;
- s is an integer between 1 and 10;
- t is an integer between 1 and 10;
- u is an integer between 1 and 10;
- and
- v is and integer between 1 and 200
- a is greater than 0;
- and
- n is ≧1.
A protein adsorption resistant surface having the formula:
- R1—(R2)m
- wherein,
- R1 is selected from organic polymers, glass and silica-based polymers;
- R2 is a hydroxyethoxylated polyethyleneimine;
- and
- m is at least 1.
A protein adsorption resistant surface having the formula:
- R1—(R2)m
- wherein,
- R1 is selected from organic polymers, glass and silica-based polymers;
- R2 has the formula
- in which:
- R5 is a member selected from the group consisting of —R6 and NR6R7, wherein
- R6 and R7 are independently members selected from the group consisting of
- t is greater than 1
- X is greater than 1;
- Y is greater than 1;
- and
- Z is greater than 1;
- and
- m is at least 1.
A method of manufacturing a protein adsorption resistant microfluidic device having at least one microchannel therein, said microchannels having a surface, said method comprising:
- (a) derivatizing said surface of said microchannel with a first modifying agent;
- (b) monitoring the extent of said derivatizing by:
- (i) establishing an electroosmotic flow of a material within said channel;
- (ii) measuring a property of said flow to obtain a value;
- and,
- (iii) comparing said value to a reference value for said property, thereby monitoring said extent of said providing.
A method of manufacturing a protein adsorption resistant microfluidic device having at least one microchannel therein, said microchannels having a surface coating comprising n subunits, said method comprising:
- (a) derivatizing said surface of said microchannel with a surface modifying agent;
- (b) monitoring the extent of said derivatizing by:
- (i) establishing an electroosmotic flow of a material within said channel;
- (ii) measuring a property of said flow to obtain a value;
- and,
- (iii) comparing said value to a reference value for said property, thereby monitoring said extent of said providing;
- and;
- (c) repeating steps (a) and (b) n−1 times.
Dependencies are inferred from the claim text.