Grafts made from amniotic membrane; methods of separating, preserving, and using such grafts in surgeries
OwnerScheffer C. G. Tsenginventor
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Abstract
A method for making, storing and using a surgical graft from human amniotic membrane; the resulting graft; and the storage solution. The amniotic membrane is obtained from human placenta, from which the chorion has been separated. Sheets of the amniotic membrane are cut to size and mounted on filter paper. The cells of the amniotic membrane are killed, preferably while being frozen and thawed in the storage solution. The storage solution comprises a culture medium and a hyperosmotic agent, wherein the hydration of the amniotic membrane is maintained. The membrane can be impregnated with therapeutic agents, prior to storage, for use in post surgical healing or other therapies.
Claims48
A surgical graft comprising amniotic membrane prepared by a method comprising:
- separating an amniotic membrane of a human placenta from its chorion, said amniotic membrane comprising cells and an extracellular matrix;
- mounting said amniotic membrane onto a substrate, with the epithelial surface of said amniotic membrane facing away from said substrate;
- and
- killing said cells of said amniotic membrane while maintaining the integrity of said extracellular matrix, prior to using said amniotic membrane as a surgical graft.
A surgical graft comprising amniotic membrane prepared by a method comprising:
- separating an amniotic membrane of a human placenta from its chorion, said amniotic membrane comprising cells and an extracellular matrix;
- mounting said amniotic membrane onto a substrate, with the epithelial surface of said amniotic membrane facing away from said substrate;
- killing said cells of said amniotic membrane while maintaining the integrity of said extracellular matrix, by freezing said amniotic membrane in a liquid comprising a culture medium and a hyperosmotic reagent;
- and
- thawing said amniotic membrane prior to using said amniotic membrane as a surgical graft.
A surgical graft comprising:
- human amniotic membrane comprising cells and an extracellular matrix, wherein said cells have been killed prior to said amniotic membrane being used for a surgical graft.
A storage solution for a surgical graft comprising amniotic membrane, comprising;
- a culture medium;
- and
- a hyperosmotic agent.
Dependencies are inferred from the claim text.