Reagents and methods for releasing and measuring lead ions from biological matrices
AssigneeBayer Aktiengesellschaft
InventorsEddy Chapoteau, Bronislaw P. Czech, Jonathan Craine
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Abstract
The present invention provides a reagent for extracting lead ions from a variety of biological matrices such as human whole blood that has been anticoagulated with either heparin or EDTA, and lyophilized human blood and bovine EDTA whole blood. The released lead is measured in situ with a calorimetric porphyrin reagent without further manipulation of the supernatant. The lead ion releasing reagent can be used in conjunction with a variety of assay formats for the determination of lead in various biological fluids.
Claims16
A colorimetric reagent adapted for the spectrophotometric analysis of a solution of lead ions extracted from a biological matrix with a releasing reagent, wherein said colorimetric reagent is a solution which consists essentially of a colorimetric porphyrin compound adapted to exhibit a spectral shift to enable the detection of lead by spectrophotometric analysis and sufficient amounts of a first additive comprising a quatemary ammonium salt adapted to increase the sensitivity of said colorimetric reagent and improve the spectral response, and sufficient amounts of a second additive consisting of ammonium hydroxide adapted to mask interfering cations selected from the group consisting of copper ions and zinc ions which may be present in the solution of lead ions.
Dependencies are inferred from the claim text.