Adipogenic differentiation of human mesenchymal stem cells
AssigneeOsiris Therapeutics, Inc.
InventorsMark F. Pittenger, Stephen C. Beck
Life of this patent
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Abstract
A composition which comprises human mesenchymal stem cells which have the potential to differentiate into cells of more than one connective tissue type and a composition which induces cells from the mesenchymal stem cell population to differentiate into the adipogenic lineage, and a process for inducing such differentiation. The composition for inducing such differentiation comprises a glucocorticoid, a compound which stimulates cAMP production or inhibits cAMP degradation (such as a phosphodiesterase inhibitor), and/or a compound which upregulates peroxisome proliferator activated receptor γ (PPAR γ) expression and/or increases its binding affinity to its DNA binding site. The process can further include isolating the adipocytes from remaining hMSCs.
Claims12
A composition which comprises human mesenchymal stem cells (hMSCs) and a substance which induces cells from the mesenchymal stem cell population to differentiate into the adipogenic lineage wherein the substance comprises (i) a glucocorticoid;
- (ii) a member selected from the group consisting of (a) a compound which elevates intracellular levels of cAMP and (b) a compound which inhibits degradation of cAMP;
- and (iii) a compound which upregulates peroxisome proliferator activated receptor γ (PPAR γ) expression and/or increases its binding affinity to its DNA binding site.
A process for inducing a human mesenchymal stem cells (hMSCs) to differentiate into adipocytes, comprising:
- contacting human mesenchymal stem cells with (i) a glucocorticoid;
- (ii) a member selected from the group consisting of (a) a compound which elevates intracellular levels of cAMP and (b) a compound which inhibits degradation of cAMP;
- and (iii) a compound which upregulates peroxisome proliferator activated receptor 65 (PPAR γ) expression and/or increases its binding affinity to its DNA binding site in an amount sufficient to induce human mesenchymal stem cells to differentiate into adipocytes.
Dependencies are inferred from the claim text.