Back to results
US 6,320,103 B1Granted
Polypeptides having choline oxidase activity and nucleic acids encoding same
AssigneeNovozymes Biotech, Inc.
InventorsDebbie Yaver, Randy M. Berka, Michael W. Rey
Life of this patent
Estimating the term…
Abstract
The present invention relates to isolated polypeptides having choline oxidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Claims12
A transgenic plant having enhanced osmotolerance comprising a nucleic acid construct comprising a nucleic acid sequence encoding a polypeptide having choline oxidase activity obtained from Fusarium venenatum, selected from the group consisting of:
- (a) a nucleic acid sequence encoding a polypeptide having an amino acid sequence which has at least 95% identity with amino acids 1 to 543 of SEQ ID NO:2;
- (b) a nucleic acid sequence having a sequence which has at least 95% homology with nucleotides 49 to 1677 of SEQ ID NO:1;
- and
- (c) a nucleic acid sequence, encoding a polypeptide having choline oxidase activity, which hybridizes under low stringency conditions with (i) nucleotides 49 to 1677 of SEQ ID NO:1, (ii) a sequence of (i) of at least 100 contiguous nucleotides, or (iii) a complementary strand of (i) or (ii), wherein low stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 25% formamide, and washing with 2×SSC, 0.2% SDS at 50° C.
1 more element
Dependencies are inferred from the claim text.