US 6,320,100 B1Granted

Synthetic DNA sequences having enhanced insecticidal activity in maize

AssigneeSyngenta Investments, Inc.

InventorsMichael G. Koziel, Nalini M. Desai, Kelly S. Lewis, Gregory W. Warren, Stephen V. Evola, Martha S. Wright, Karen L. Launis, Steven J. Rothstein, Cindy G. Bowman, John L. Dawson, Erik M. Dunder, Gary M. Pace, Janet L. Suttie

Life of this patent

Estimating the term…

patentgrep

Loading patent…

Abstract

DNA sequences optimized for expression in plants are disclosed. The DNA sequences preferably encode for an insecticidal polypeptides, particularly insecticidal proteins from Bacillus thuringiensis. Plant promoters, particular tissue-specific and tissue-preferred promoters are also provided. Additionally disclosed are transformation vectors comprising said DNA sequences. The transformation vectors demonstrate high levels of insecticidal activity when transformed into maize.

Claims34

Dependent claims

A synthetic DNA coding sequence that encodes a Bacillus thuringiensis (Bt) insecticidal protein selected for optimized expression in a plant, wherein said synthetic DNA coding sequence is produced by a method comprising:

(a) obtaining the amino acid sequence of said Bt insecticidal protein;
(b) reverse-translating said amino acid sequence into a synthetic DNA coding sequence comprising a sufficient number of the single codons that most frequently encode each amino acid in maize, wherein said synthetic DNA coding sequence has at least about 60% G+C content, and wherein the single codons that most frequently encode each amino acid in maize are determinable by
(i) pooling a plurality of gene sequences from maize,
(ii) calculating a codon usage profile from said pooled maize gene sequences, and
(iii) determining which single codon most frequently encodes each amino acid in maize;
and
(c) synthesizing said DNA coding sequence.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 09/547,422Filed 11 Apr 2000 · continuation
Priority: 4 Oct 1991
Granted: 20 Nov 2001
Term ends: Not estimatedThe source records don’t support an estimate.

Classification8 codes

C07K 14/325
From Bacillus: bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
C12N 9/12
Transferases: transferring phosphorus containing groups, e.g. kinases
C12N 9/88
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: lyases
C12N 15/8286
For biotic stress resistance, pathogen resistance, disease resistance: for insect resistance

Family

Application07/772,027Parent of this continuation-in-part · filed 4 Oct 1991
Application07/951,715Parent of this division · filed 25 Sep 1992
Application08/459,504Parent of this continuation · filed 2 Jun 1995
US 6,075,185 AParent of this continuation
Application08/459,504Parent of this division
US 5,625,136 AParent of this division
Application07/951,715Parent of this continuation-in-part
US 6,320,100 B1This patent

From the US filing history. Foreign family members aren’t listed.