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US 6,320,041 B1Granted
Pre-activated carbonyl linkers for the modification of oligonucleotides
AssigneeTriLink BioTechnologies, LLC
InventorsRichard I. Hogrefe, Morteza M. Vaghefi
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Abstract
The present invention provides a variety of compositions that comprise an activated carboxylic ester moiety on one end of an alkyl tether and a nucleic acid binding moiety on the other end for use as crosslinking reagents in conjugation reactions of nucleotides with solid support matrixes, organic molecules, reporter groups or other biomolecules.
Claims25
A composition of the formula,
- wherein
- R1 is
- R3 is —CH3, —O—CH2—CH2—CN or —O—CH3;
- R4 is o-chlorophenyl, β-cyanoethyl;
- X1 is O, S or Se;
- and
- n is 5 to 15.
4 more elements
A composition of the formula,
- wherein
- R1 is
- R2 is —CH3, —O—CH2—CH2—CN or —O—CH3;
- X is O, S or Se;
- and
- n is 5to 15.
3 more elements
A composition of the formula,
- wherein
- R5 is a reporter group, a biomolecule, an organic molecule, or a nucleic acid not less than 1 nucleotide in length and a nucleic acid not more than 1,000 nucleotides in length;
- R6 is a nucleic acid not less than 1 nucleotide in length and a nucleic acid not more than 1,000 nucleotides in length;
- X1 is O, S, or Se;
- and
- n is a number not less than 5 and a number not more than 15.
3 more elements
A composition of the formula,
- wherein
- R5 is a reporter group, a biomolecule, an organic molecule, or a nucleic acid not less than 1 nucleotide in length and a nucleic acid not more than 1,000 nucleotides in length;
- R6 is a nucleic acid not less than 1 nucleotide in length and a nucleic acid not more than 1,000 nucleotides in length;
- X1 is O, S, or Se;
- and
- n is a number not less than 5 and a number not more than 15.
3 more elements
Dependencies are inferred from the claim text.