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US 6,320,023 B1Granted
Macrophage derived chemokine
AssigneeICOS Corporation
InventorsRonald Godiska, Patrick W. Gray
Life of this patent
Estimating the term…
Abstract
The present invention provides purified and isolated polynucleotide sequences encoding a novel human macrophage-derived C-C chemokine designated MDC. Also provided are materials and methods for the recombinant production of the chemokine, and purified and isolated chemokine protein.
Claims3
A purified polypeptide consisting of amino acids 25 to 93 of SEQ ID NO:
- 2.
A purified polypeptide comprising amino acids 25 to 93 of SEQ ID NO:
- 2, wherein amino acid 25 (glycine) of SEQ ID NO:
- 2 is the amino terminus of tle polypeptide.
A purified polypeptide, wherein the polypeptide:
- has an amino terminus consisting of the sequence:
- Gly-Pro-Tyr-Gly-Ala-Asn-Met-Glu-Asp-Ser-Val-Cys-Cys-Arg-Asp (SEQ ID NO:
- 2, positions 25-39);
- and
- has a molecular weight of about 7 kD as assessed by denaturing SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE);
- and
- is encoded by a polynucleotide isolatable from a human macrophage cDNA library via polymerase chain reaction (PCR) using a primer pair selected from the group consisting of:
- (a) Primers 390-1F and 390-2R (SEQ ID NOS:
- 7 and 8);
- (b) Primers 390-1F and 390-4R (SEQ ID NOS:
- 7 and 9);
- (c) Primers 390-FX2 and 390-2R (SEQ ID NOS:
- 11 and 8);
- and
- (d) Primers 390-Pel and 390-2R (SEQ ID NOS:
- 13 and 8).
13 more elements
Dependencies are inferred from the claim text.