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AssigneesChiron Corporation, G.D. Searle & Co.
InventorsGlenn J. Dorin, Bo H. Arve, Gregory L. Pattison, Robert F. Halenbeck, Kirk Johnson, Bao-Lu Chen, Rajsharan K. Rana, Maninder S. Hora, Hassan Madani, Michael Tsang, Mark E. Gustafson, Gary S. Bild, Gary V. Johnson
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A method of modifying protein solubility employs polyionic polymers. These facilitate the solubilization, formulation, purification and refolding of proteins especially incorrectly folded proteins and aggregated proteins. Compositions are described that are suitable for formulating TFPI. The compositions allow preparation of pharmaceutically acceptable compositions of TFPI at concentrations above 0.2 mg/mL and above 10 mg/mL.
A method of modifying the solubility of a first polymer consisting of a protein having a first domain which has a net positive charge and a second domain which has a net negative charge, comprising the steps of:
A method of refolding an improperly folded or denatured protein comprising the step of adding a charged polymer to a solution comprising said protein in the absence of urea prior to allowing said protein to refold.
Dependencies are inferred from the claim text.