US 6,319,726 B1 · Immunological method for detecting antibodies directed against tissue transglutaminase (tTG), use of tTG in diagnosis and therapy control, and an oral pharmaceutical agent containing tTG

Abstract

The invention relates to a method for detecting antibodies from body fluids by means of an immune reaction with tissue transglutaminase (tTG), with tTG-containing compounds, the antigenic structures, immunoreactive sequences or analogues thereof. The method may be used in the diagnosis and therapy control of diseases associated with an immune reaction against tTG, tTG-containing compounds, the antigenic structures, immunoreactive sequences or analogues thereof. Therefore, the invention is also directed to the use of tTG and the above-mentioned substances in diagnosis and therapy control, preferably in the diagnosis and therapy control of chronically inflammatory diseases or autoimmune diseases, and more preferably, in the diagnosis and therapy control of sprue or coeliac disease.

Claims14

Dependent claims

A method of identifying coeliac disease in a subject comprising the steps of:

(i) contacting a sample of body fluid from the subject with a purified target antigen, wherein the target antigen comprises an epitope of tissue transglutaminase and has the property of being specifically recognized by at least one antibody directed towards tissue transglutaminase under conditions that allow binding of an antibody to the target antigen;
and
(ii) detecting the binding of antibodies in the body fluid to the target antigen;
wherein the binding of said antibodies bears a positive correlation with the existence of coeliac disease in the subject.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

09/229,716Filed 13 Jan 1999 · continuation

Priority

DE 196 30 55718 Jul 1996

Granted

20 Nov 2001

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 9/1044
Aminoacyltransferases: protein-glutamine gamma-glutamyltransferase , i.e. transglutaminase or factor XIII
A61P 37/00
Drugs for immunological or allergic disorders
C07K 14/4713
Not used: autoimmune diseases, e.g. Insulin-dependent diabetes mellitus, multiple sclerosis, rheumathoid arthritis, systemic lupus erythematosus;…
G01N 33/564
Immunoassay; Biospecific binding assay; Materials therefor: for pre-existing immune complex or autoimmune disease , i.e. systemic lupus…

Immunological method for detecting antibodies directed against tissue transglutaminase (tTG), use of tTG in diagnosis and therapy control, and an oral pharmaceutical agent containing tTG

Life of this patent

Immunological method for detecting antibodies directed against tissue transglutaminase (tTG), use of tTG in diagnosis and therapy control, and an oral pharmaceutical agent containing tTG

Life of this patent

Abstract

Claims14