Process for fast visualization of protein
OwnerEwald M. Wondrakinventor
Life of this patent
Estimating the term…
Abstract
The present invention provides an improved method for staining proteins in and/or on a solid matrix or support. Typically, a protein-containing gel or membrane may be washed in a hot solution of water for about five minutes, stained in a hot solution of COOMASSIE brilliant blue dye in dilute aqueous mineral acid for about five minutes, and then rinsed in water. The washing and/or staining steps may be performed by placing the gel in a wash and/or staining solution, respectively, heating the solution in which the gel is placed to boiling in a microwave oven, and incubating the gel in the solution for about five minutes. The entire procedure can be performed in a little over ten minutes, which represents an enormous time savings over existing methods.
Claims34
A method for staining a protein in a gel comprising staining the gel with a brilliant blue dye in a hydrochloric or perchloric acid solution, wherein said acid has a concentration equivalent of between 5 and 100 mM HCl, for an incubation time less than one hour and at an initial temperature of at least 45° C.
A method for staining a protein immobilized on a membrane comprising staining the membrane with a brilliant blue dye in a hydrochloric or perchloric acid solution, wherein said acid has a concentration less than 100 mM, for an incubation time less than 15 minutes.
A method for staining a protein in a gel comprising:
- (a) washing the gel with a wash solution prior to staining the gel;
- (b) staining the gel with a staining solution for an incubation time less than 15 minutes and at an initial temperature of at least 70° C., wherein said staining solution contains colloidal brilliant blue dye in a dilute acid solution, said dilute acid has a concentration less than 100 mM, said brilliant blue dye has a concentration between 0.0005% (w/v) and 0.5% (w/v), and said brilliant blue dye is at least one of brilliant blue G and brilliant blue R;
- and
- (c) rinsing the gel to remove brilliant blue dye subsequent to staining the gel.
A method of staining protein comprising:
- (a) providing protein immobilized in and/or on a solid matrix, and
- (b) staining said protein with a solution comprising at least one brilliant blue dye as colloids in a dilute acid solution;
- wherein said dilute acid has a concentration equivalent of between about 1 mM and 100 mM, and said at least one brilliant blue dye has a concentration between 0.0005% (w/v) and 0.5% (w/v).
Dependencies are inferred from the claim text.