US 6,319,716 B1 · Bovine adenovirus type 3 genome and vector systems derived therefrom

Abstract

The present invention provides the complete nucleotide sequence of a bovine adenovirus. The invention further provides bovine adenovirus vectors and expression systems which can be used, among other things, for insertion of foreign sequences, for provision of DNA control sequences including transcriptional and translational regulatory sequences, for diagnostic purposes to detect the presence of viral nucleic acids or proteins encoded by these regions in a subject or biological sample, for provision of immunogenic polypeptides or fragments thereof, for vaccines and for gene therapy. Cell lines comprising the vectors of the invention, and methods for making bovine adenovirus vectors are also provided.

Claims35

Dependent claims

A method for constructing a recombinant BAV3 vector containing a heterologous sequence inserted into an insertion site, the method comprising:

(a) linking the heterologous sequence to sequences which are substantially homologous to BAV3 sequences surrounding the insertion site, to form an insertion cassette;
wherein said insertion site is located in a region of the BAV3 genome consisting of nucleotides 4092 through 5234;
nucleotides 5892 through 17,735;
nucleotides 21,198 through 26,033;
or nucleotides 31,133 through 34,445 of FIGS. 20:1-20:19 (SEQ ID NO:
35);
(b) introducing the insertion cassette into a cell, along with a polynucleotide comprising a sequence that is substantially homologous to a BAV3 genome;
and
(c) allowing homologous recombination to occur between the insertion cassette and the polynucleotide to generate the recombinant BAV3 vector.

6 more elements

Dependencies are inferred from the claim text.

Bovine adenovirus type 3 genome and vector systems derived therefrom

Life of this patent

Bovine adenovirus type 3 genome and vector systems derived therefrom

Life of this patent

Abstract

Claims35