Recombinant virus vectors
OwnerPeter G. Speckinventor
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Abstract
A mutant herpesvirus that can be used a recombinant virus vector includes (a) a mutation such that the mutant virus has a reduced ability in comparison with a parent type to cause lysis of an infected cell, and (b) an inactivating mutation in a gene essential for the production of infectious virus. An example is a HSV1 mutant lacking the essential glycoprotein gH gene and having a mutation impairing the function of the gene product VP16. A heterologous gene can be carried at the site of the inactivated essential gene, e.g. a gene suitable for administering gene therapy. The vector has an increased margin of safety over known herpesvirus vectors in respect of incidence of cytopathic effects and/or risk of infection.
Claims9
A mutant herpesvirus comprising (a) a first mutation such that the virus has a reduced ability in comparison with a parent type to cause lysis of an infected cell, and (b) a second inactivating mutation in an endogenous gene which is essential for the production of infectious virus but is not essential for viral DNA replication,
- wherein the mutation (a) is a mutation effective to reduce the ability of the virus to cause expression of at least one immediate early gene function.
A mutant herpesvirus comprising (a) a first mutation such that the virus has a reduced ability in comparison with a parent type to cause lysis of an infected cell, and (b) a second inactivating mutation in an endogenous gene which is essential for the production of infectious virus but is not essential for viral DNA replication, wherein the herpesvirus is a herpes simplex virus of type 1 (HSV-1) or a herpes simplex virus of type 2 (HSV-2) and wherein mutation (a) is a mutation in a gene encoding ICP0.
A mutant herpesvirus comprising (a) a first mutation such that the virus has a reduced ability in comparison with a parent type to cause lysis of an infected cell, and (b) a second inactivating mutation in an endogenous gene which is essential for the production of infectious virus but is not essential for viral DNA replication, wherein the herpesvirus is a herpes simplex virus of type 1 (HSV-1) or a herpes simplex virus of type 2 (HSV-2) and wherein mutation (a) is a mutation in a gene encoding ICP4.
A mutant herpesvirus comprising (a) a first mutation such that the virus has a reduced ability in comparison with a parent type to cause lysis of an infected cell, and (b) a second inactivating mutation in an endogenous gene which is essential for the production of infectious virus but is not essential for viral DNA replication, wherein the herpesvirus is a herpes simplex virus of type 1 (HSV-1) or a herpes simplex virus of type 2 (HSV-2) and wherein mutation (a) is a mutation in a gene encoding ICP22.
A mutant herpesvirus comprising (a) a first mutation such that the virus has a reduced ability in comparison with a parent type to cause lysis of an infected cell, and (b) a second inactivating mutation in an endogenous gene which is essential for the production of infectious virus but is not essential for viral DNA replication, wherein the herpesvirus is a herpes simplex virus of type 1 (HSV-1) or a herpes simplex virus of type 2 (HSV-2) and wherein mutation (a) is a mutation in a gene encoding ICP27.
Dependencies are inferred from the claim text.