Cloning of chicken anemia virus DNA
AssigneeLeadd B.V.
InventorsMatheus H. M. Noteborn, Gerben F. de Boer
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Abstract
Recombinant genetic information (DNA or RNA), comprising a Chicken Anemia Virus (CAV)-specific nucleotide sequence and the use thereof for diagnostics, vaccination or protein production. Recombinant CAV protein and the use thereof for diagnostics, vaccination or production of CAV-specific antibodies. The use of CAV-specific antibodies thus obtained.
Claims44
A recombinant nucleic acid comprising:
- a first nucleotide sequence, wherein said first nucleotide sequence is a full length CAV genome, and wherein said recombinant nucleic acid is functional to produce infectious virus in the absence of pre-existing viral protein.
A recombinant nucleic acid comprising:
- a first nucleotide sequence, wherein said first nucleotide sequence is a part of the nucleotide sequence of a CAV genome, and wherein said first nucleotide sequence encodes a CAV protein.
A recombinant nucleic acid comprising:
- a first nucleotide sequence, wherein said nucleic acid is RNA and wherein said first nucleotide sequence is a full length CAV genome or is 100% complementary to a full length CAV genome.
The recombinant nucleic acid according to claim 11, wherein said RNA is labelled with a marker selected from the group consisting of radioisotopes, enzyme molecules, haptens, fluorescent substances, dyes, pigments, and particulate markers.
A diagnostic kit, comprising:
- a nucleic acid primer pair, wherein the sequence of each nucleic acid of said primer pair is a CAV-specific nucleotide sequence of a CAV genome or part thereof or is 100% complementary to a CAV-specific nucleotide sequence of a CAV genome.
A reagent for diagnosing the presence of a chicken anemia virus (CAV) nucleotide sequence, said reagent consisting of:
- a single stranded linear nucleic acid that specifically hybridizes to only a predetermined CAV-specific fragment of the nucleotide sequence of SEQ ID NO:1.
A recombinant nucleic acid, which is a CAV-specific fragment of a CAV genome comprising:
- the nucleotide sequence of SEQ ID NO:26 or a sequence 100% complementary thereto.
A recombinant nucleic acid, which is a CAV-specific fragment of a CAV genome comprising:
- the nucleotide sequence of SEQ ID NO:27 or a sequence 100% complementary thereto.
A recombinant nucleic acid, which is a CAV-specific fragment of a CAV genome comprising:
- the nucleotide sequence of SEQ ID NO:28 or a sequence 100% complementary thereto.
A recombinant nucleic acid, which is a CAV-specific fragment of a CAV genome comprising:
- the nucleotide sequence of SEQ ID NO:23 or a sequence 100% complementary thereto.
A recombinant nucleic acid, which is a CAV-specific fragment of a CAV genome comprising:
- the nucleotide sequence of SEQ ID NO:24 or a sequence 100% complementary thereto.
A recombinant nucleic acid, which is a CAV-specific fragment of a CAV genome comprising:
- the nucleotide sequence of SEQ ID NO:25 or a sequence 100% complementary thereto.
A recombinant nucleic acid comprising:
- the nucleotide sequence of SEQ ID NO:1, free of cellular components.
A method of transfecting avian cells, said method comprising:
- providing an avian cell with a vector comprising a nucleotide sequence of SEQ ID NO:1 under conditions wherein said avian cell is transfected.
An oligonucleotide probe or primer which is a CAV-specific fragment of a CAV genome comprising at least 19 bases.
An isolated CAV genome, wherein said genome comprises:
- one each of the restriction endonuclease cleavage sites BglI-657, SstI-863, AccI-974, BglI-1 163, BamHI-1507, HindIII-1757, and XbaI-1849.
A method of amplifying a region of a CAV nucleic acid, said method comprising:
- contacting said CAV nucleic acid with a forward and a reverse primer;
- and performing cycles of melting, reannealing, and DNA synthesis, whereby said region of a CAV nucleic acid is amplified.
A diagnostic kit for detecting chicken anemia virus (CAV) RNA, wherein said diagnostic kit comprises:
- a linear nucleic acid, wherein the sequence of said linear nucleic acid is a defined CAV-specific fragment of a CAV genome or part thereof or is 100% complementary to a CAV-specific fragment of a CAV genome or part thereof.
A method ot producing a recombinant chicken anemia virus genome, said method comprising:
- linearizing a double stranded circular genome of a chicken anemia virus to produce a linearized genome;
- and
- recircularizing said linearized genome, whereby said recombinant chicken anemia virus genome is produced.
Dependencies are inferred from the claim text.