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AssigneeKabushiki Kaisha Yakult Honsha
InventorsMariko Kadota, Mayumi Kiwaki, Saeko Sawaki, Yukio Shirasawa, Harue Sone, Tomoyuki Sako
Estimating the term…
Methods for transferring a foreign gene into a host chromosome by integrating the foreign gene into a vector by utilizing a lysogenic phage, for the purpose of deleting unnecessary genes derived from the vector, except for the foreign gene. One method comprises (a) preparing a vector with a lysogenic phage integration site (such as an attP site) arranged between a first partial sequence of the objective foreign gene to be transferred, but lacking one terminal region thereof and a second partial sequence of the gene, but lacking the other terminal region, the second partial sequence having an overlapping region with a portion of the first partial sequence; (b) integrating the vector obtained in step (a) into the host chromosome; and (c) screening from among the recombinants obtained in step (b) a recombinant from which unnecessary genes originating in the vector have been deleted owing to the homologous recombination mechanism functioning in the overlapping region between the first and second partial sequences.
A method for integrating a foreign gene into a bacterial host chromosome comprising:
A method for integrating a foreign gene comprising regions A, B and C, wherein the region A and the region C represent partial sequences of different termini of the foreign gene;
A Lactobacillus casei vector which permits the integration of a foreign gene into the chromosome of Lactobacillus casei, carrying:
A Lactobacillus casei vector pMSK742 carrying the sequences of the restriction enzyme recognition sites as depicted in FIG. 1.
Dependencies are inferred from the claim text.