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US 6,319,686 B1Granted
Nucleic acids encoding kappa opioid receptors
AssigneeArch Development Corporation
InventorsGraeme I. Bell, Terry Reisine, Kazuki Yasuda
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AssigneeArch Development Corporation
InventorsGraeme I. Bell, Terry Reisine, Kazuki Yasuda
Estimating the term…
The invention relates generally to compositions of and methods for obtaining opioid receptor polypeptides. The invention relates as well to polynucleotides encoding opioid receptor polypeptides. More specifically, the invention relates to polynucleotides encoding kappa opioid receptor polypeptides, the recombinant vectors carrying those sequences, the recombinant host cells including either the sequences or vectors, and recombinant opioid receptor polypeptides. By way of example, the invention discloses the cloning and functional expression of at least three different opioid receptor polypeptides. The invention includes as well, methods for using the isolated, recombinant receptor polypeptides in assays designed to select and improve substances capable of interacting with opioid receptor polypeptides for use in diagnostic, drug design and therapeutic applications.
An isolated and purified nucleic acid comprising a base sequence that is identical or complementary to a segment of at least 30 contiguous bases of SEQ ID NO:1, wherein said nucleic acid hybridizes, under hybridization conditions employing between 0.02 M and 0.15 M NaCl at a temperature between 50° C. and 70° C., to a nucleic acid that encodes a mammalian kappa opioid receptor polypeptide or to the complement of such a sequence.
A process for preparing a mammalian kappa opioid receptor polypeptide in a recombinant host cell, comprising the steps of:
An isolated and purified nucleic acid comprising a base sequence that is identical or complementary to a segment of at least 30 contiguous bases of SEQ ID NO:1.
An expression vector comprising a nucleic acid that comprises a base sequence that is identical or complementary to at least 30 contiguous bases of SEQ ID NO:1.
A recombinant host cell comprising nucleic acid that comprises a base sequence that is identical or complementary to at least 30 contiguous bases of SEQ ID NO:1.
An isolated and purified nucleic acid comprising a base sequence that is identical or complementary to 30 contiguous bases of SEQ ID NO:11.
An expression vector comprising a nucleic acid segment comprising at least 40 contiguous bases of SEQ ID NO:1.
An expression vector comprising a nucleic acid segment consisting of 40 contiguous bases of SEQ ID NO:11.
A recombinant host cell comprising a nucleic acid segment comprising a nucleic acid sequence consisting of 40 contiguous bases of SEQ D NO:11.
Dependencies are inferred from the claim text.