Method for analyzing monosaccharide in a sugar composition
AssigneesHonen Corporation, Zaidanhojin Sugiyama Sangyo Kagaku
InventorsShoichi Yasuno, Masugu Kamei, Junko Arai, Keiko Saito
Life of this patent
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Abstract
A method for the determination of monosaccharides in a sugar composition by simultaneous quantitative analysis of monosaccharides constituting the sugar composition, comprising the steps of: (1) liberating sialic acid from the sugar composition with sialidase or an acid, (2) converting the released sialic acid into N-acylmannosamine with sialic acid aldolase, and (3) acid hydrolyzing the N-acylmannosamine and a sugar residue. The method allows pretreatment in a single reactor and the sugar composition including sialic acid can be obtained by a single analysis by HPLC, etc.
Claims24
A method of identifying monosaccharides present in a sugar-containing compound, and quantitatively analyzing the amounts of the respective monosaccharides present, comprising:
- contacting a sugar-containing compound with sialidase or acid thereby producing a first mixture comprising sialic acid and a sialidase-treated sugar-containing compound or acid-treated sugar-containing compound;
- converting the produced sialic acid into N-acylmannosamine by contacting the produced sialic acid with sialic acid aldolase, thereby producing a second mixture comprising N-acylmannosamine and a sialidase-treated or acid-treated sugar-containing compound;
- acid hydrolyzing the second mixture;
- and
- analyzing the resulting acid hydrolyzate to determine the identities and amounts of monosaccharides present in the acid hydrolyzate.
A method of identifying monosaccharides present in a sugar-containing compound, and quantitatively analyzing the amounts of the respective monosaccharides present, comprising:
- contacting a sugar-containing compound with sialidase or acid, thereby producing a first mixture comprising sialic acid and a sialidase-treated sugar-containing compound or acid-treated sugar-containing compound;
- converting the produced sialic acid into N-acylmannosamine by contacting the produced sialic acid with sialic acid aldolase, thereby producing a second mixture comprising N-acylmannosamine and a sialidase-treated or acid-treated sugar-containing compound;
- acid hydrolyzing the second mixture;
- N-acetylating de-N-acylated monosaccharides present in the resulting acid hydrolyzate, thereby producing a third mixture;
- and
- analyzing the third mixture to determine the identities and amounts of monosaccharides present the third mixture.
A method of identifying monosaccharides present in a sugar-containing compound and quantitatively analyzing the amounts of the respective monosaccharides present comprising:
- contacting a sugar-containing compound with sialidase or acid, thereby producing a first mixture comprising sialic acid and a sialidase-treated sugar-containing compound or acid-treated sugar-containing compound;
- converting the produced sialic acid into N-acylmannosamine by contacting the produced sialic acid with sialic acid aldolase, thereby producing a second mixture comprising N-acylmannosamine and a sialidase-treated or acid-treated sugar-containing compound;
- acid hydrolyzing the second mixture;
- N-acetylatiing de-N-acylated monosaccharides present in the resulting acid hydrolyzate, thereby producing a third mixture;
- labeling monosaccharides present in the third mixture with ethyl 4-aminobenzoate (ABEE), thereby creating a fourth mixture;
- and
- analyzing the fourth mixture to determine the identities and amounts of monosacchrides present in the acid hydrolyzate.
Dependencies are inferred from the claim text.