Purification of a triple helix formation with an immobilized oligonucleotide
AssigneeAventis Pharma S.A.
InventorsJoël Crouzet, Daniel Scherman, Pierre Wils, Françis Blanche, Béatrice Cameron
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Abstract
Method for double-stranded DNA purification, by which a solution containing said DNA in a mixture with other components is passed over a support on which is covalently coupled in oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix.
Claims21
A method for purifying double-stranded DNA from a solution containing the double-stranded DNA mixed with other components, comprising passing the solution through a support comprising a covalently coupled oligonucleotide capable of forming a triple helix with the double-stranded DNA by hybridization with a specific sequence present in the double-stranded DNA, wherein the covalently coupled oligonucleotide comprises the sequence TCTTTTTTTCCT (SEQ ID NO:28) or TTCTTTTTTTTCTT (SEQ ID NO:30).
A method for purifing double-stranded DNA from a solution containing the double-stranded DNA mixed with other components, comprising passing the solution through a support comprising a covalently coupled oligonucleotide capable of forming a triple helix with the double-stranded DNA by hybridization with a specific sequence present in the double-stranded DNA, wherein the specific sequence present in the double-stranded DNA comprises the sequence AGAAAAAAAGGA (SEQ ID NO :27) or AAGAAAAAAAAGAA (SEQ ID NO:29).
A method for purifying double-stranded RNA from a solution containing the double-stranded RNA mixed with other components, comprising passing the solution through a support comprising a covalently coupled oligonucleotide capable of forming a triple helix with the double-stranded RNA by hybridization with a specific sequence present in the double-stranded RNA, wherein the oligonucleotide comprises the sequence TCTTTTTTTCCT (SEQ ID NO:28) or TTCTTTTTTTTCTT (SEQ ID NO:30).
Dependencies are inferred from the claim text.