Compositions and methods for determining predisposition to insulin dependent diabetes mellitus
AssigneeSaigene Corporation
InventorsJack U'ren, Sharon Howard, Vivian H. Gersuk
Life of this patent
Estimating the term…
Abstract
Methods for detecting in a human biological sample the presence of HLA DQB1 alleles 0201/0202, 0302/0303, and/or 0602 which are associated with increased or decreased susceptibility to insulin dependent diabetes mellitus.
Claims16
A method of detecting the presence of HLA DQB1 alleles 0201/0202, 0302/0303, and 0602 in a human biological sample, comprising the steps:
- (a) contacting, under stringent conditions, an amplicon comprising a sequence from codon 23 through 41 of the human leukocyte antigen (HLA) DQB1 gene with at least three unique human leukocyte antigen (HLA) DQB1 allele-specific probes to form a hybridization complex, wherein:
- said amplicon is no more than 2000 base pairs in length and is amplified with at least two primers, wherein one of said two primers selectively hybridizes, under stringent conditions, to the same nucleic acid sequence of the HLA DQB1 allele as a primer having the sequence 5′-GGAGCGCGTGCGTCTTGTG-3′ (SEQ ID NO:7);
- said HLA DQB1 allele-specific probes is between 15 and 200 nucleotides in length;
- and
- wherein each of said DQB1 allele-specific probes includes a nucleic acid segment of at least 15 contiguous nucleotides which selectively hybridizes under stringent conditions to the same nucleic acid sequence of the HLA DQB1 allele as an oligonucleotide selected from the group consisting of:
- for the 0201 and 0202 alleles,
- 5′-AAGAGATCGTGCGCTTCGACA-3′ (SEQ ID NO:2),
- for the 0302 and 0303 alleles
- 5′-AGGAGTACGCACGCTTCGACA-3′ (SEQ ID NO:4),
- for the 0602 allele
- 5′-GGAGTACGCGCGCTTCGAC-3′ (SEQ ID NO:6),
- and complementary sequences thereof, with the proviso that each of said allele-specific probes selectively hybridizes under the same stringent hybridization conditions;
- and
- (b) detecting said hybridization complexes as an indication of the presence of said HLA DQB1 alleles 0201/0202, 0302/0303, and 0602.
A method of detecting the presence of HLA DQB1 alleles 0201/0202, 0302/0303, and 0602 in a human biological sample, comprising the steps:
- (a) contacting an amplicon comprising a sequence from codon 23 through 50 of the human leukocyte antigen (HLA) DQB1 gene with at least three unique human leukocyte antigen (HLA) DQB1 allele-specific probes to form a hybridization complex, wherein:
- said amplicon is no more than 2000 base pairs in length and is amplified with at least two primers, wherein one of said two primers selectively hybridizes, under stringent conditions, to the same nucleic acid sequence of the HLA DQB1 allele as a primer having the sequence 5′-GGAGCGCGTGCGTCTTGTG-3′ (SEQ ID NO:7);
- said HLA DQB1 allele-specific probes is between 15 and 200 nucleotides in length;
- and
- wherein each of said DQB1 allele-specific probes includes a nucleic acid segment of at least contiguous nucleotides which selectively hybridizes under stringent conditions to the same nucleic acid sequence of the HLA DQB1 allele as an oligonucleotide selected from the group consisting of:
- for the 0201 and 0202 alleles
- 5′-CGTGGGGGAGTTCCGGGCGGTG-3′ (SEQ ID NO:1),
- 5′-AAGAGATCGTGCGCTTCGACA-3′ (SEQ ID NO:2),
- for the 0302 and 0303 alleles
- 5′-CGTGGGGGTGTATCGGGCGGTG-3′ (SEQ ID NO:3),
- 5′-AGGAGTACGCACGCTTCGACA-3′ (SEQ ID NQ:4),
- for the 0602 allele
- 5′-CGTGGGGGTGTACCGCGCGGTG-3′ (SEQ ID NO:5),
- 5′-GGAGTACGCGCGCTTCGAC-3′ (SEQ ID NO:6),
- and complementary sequences thereof, with the proviso that each of said allele-specific probes selectively hybridizes under the same stringent hybridization conditions;
- and
- (b) detecting said hybridization complexes as an indication of the presence of said HLA DQB1 alleles 0201/0202, 0302/0303, and 0602.
A kit for detecting the presence of HLA DQB1 alleles 0201/0202, 0302/0303, and 0602 in a human biological sample, comprising at least three probes of 15 to 100 nucleotides in length, said probes comprising a nucleic acid segment which is complementary to the same nucleic acid sequence of the HLA DQB1 allele as an oligonucleotide selected from the group consisting of:
- SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, and complementary sequences thereof.
Dependencies are inferred from the claim text.