Methods and apparatus for improved luminescence assays using microparticles
AssigneeMeso Scale Technology LLP
InventorsGeorge B. Sigal, Jacob N. Wohlstadter, Satyanarayana Gudibande, Mark T. Martin, James L. Wilbur
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Abstract
The present invention relates to methods, reagents and compositions, for conducting electrochemiluminescence binding assays which improve one or more characteristics of the assay or the instruments used to conduct the assay. The method is achieved using microparticles that include electrically conductive material. The electrically conductive material has one or more copies of an assay ligand immobilized an its outer surface and a plurality of electrochemiluminescent moieties immobilized an its outer surface. The assay ligand may be linked to the electrochemiluminescent moiety.
Claims44
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with
- (ii) an assay-ligand immobilized on an electrode;
- and
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface and a plurality of electrochemiluminescent moieties immobilized on its surface;
- and
- (ii) an assay-ligand immobilized on an electrode;
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) a microparticle comprised of an electrically conductive material having a plurality of copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand immobilized on an electrode and being labeled with an electrochemiluminescent moiety;
- and
- (ii) an assay-ligand immobilized on an electrode;
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) said analyte,
- (ii) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte;
- and
- (iii) an assay-ligand immobilized on an electrode
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) said analyte,
- (ii) a microparticle comprised of an electrically conductive material having one or more copies of a binding reactant immobilized on its surface, said binding reactant being specific for said analyte, and
- (ii) a binding reactant immobilized on an electrode;
- and
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) said analyte,
- (ii) a microparticle comprised of an electrically conductive material having a plurality of copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand immobilized on an electrode and being labeled with an electrochemiluminescent moiety;
- an d
- (iii) an assay-ligand immobilized on an electrode.
- (b) conducing an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) said analyte,
- ii) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with (iii);
- and
- (iii) an assay-ligand immobilized on an electrode;
- and
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) said analyte,
- (ii) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface and a plurality of electrochemiluminescent moieties immobilized on its surface;
- and
- (iii) an assay-ligand immobilized on an electrode,
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) said analyte,
- (ii) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand immobilized on an electrode and being labeled with an electrochemiluminescent moiety;
- and
- (iii) an assay-ligand immobilized on an electrode;
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample comprising the steps of:
- (a) forming a composition comprising
- (i) said sample;
- and
- (ii) a microparticle comprised of an electrically conductive material having one or more of copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with the assay-ligand recited in step (c);
- (b) incubating said composition to form a complex;
- (c) causing said complex to bind to an assay-ligand immobilized on an electrode;
- and
- (d) conducting an electrochemiluminescence measurement in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample comprising the steps of:
- (a) forming a composition comprising
- (i) said sample;
- (ii) a microparticle comprised of an electrically conductive material having one or more of copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with (iii);
- and
- (iii) an assay-ligand immobilized on an electrode;
- (b) incubating said composition to form a complex;
- and
- (c) conducting an electrochemiluminescence measurement in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample comprising the steps of:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) an assay-ligand immobilized on an electrode;
- (b) incubating said system to form a complex;
- (c) causing said complex to bind to a microparticle comprised of an electrically conductive material having one or more one or more copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps of:
- (a) forming a composition comprising
- (i) said sample;
- and
- (ii) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface and a plurality of electrochemiluminescent moieties immobilized on its surface;
- (b) incubating said composition to form a complex;
- (c) causing said complex to bind to an assay-ligand immobilized on an electrode;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps of:
- (a) forming a composition comprising
- (i) said sample;
- and
- (ii) a microparticle comprised of an electrically conductive material having a plurality of copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand and being labeled with an electrochemiluminescent moiety;
- (b) incubating said composition to form a complex;
- (c) causing said complex to bind to an assay-ligand immobilized on an electrode;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface and a plurality of electrochemiluminescent moieties immobilized on its surface;
- (iii) an assay-ligand immobilized on an electrode;
- (b) incubating said system to form a complex;
- and
- (c) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) a microparticle comprised of an electrically conductive material having a plurality of copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand and being labeled with an electrochemiluminescent moiety;
- (iii) an assay-ligand immobilized on an electrode;
- (b) incubating said system to form a complex;
- and
- (c) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) an assay-ligand immobilized on an electrode;
- (b) incubating said system to form a complex;
- (c) causing said complex to bind to a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface and a plurality of electrochemiluminescent moieties immobilized on its surface;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) an assay-ligand immobilized on an electrode;
- (b) incubating said system to form a complex;
- (c) causing said complex to bind to a microparticle comprised of an electrically conductive material having a plurality of copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand and being labeled with an electrochemiluminescent moiety;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface and a plurality of electrochemiluminescent moieties immobilized on its surface;
- (b) incubating said composition to form a complex;
- (c) causing said complex to bind to an assay-ligand immobilized on an electrode;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) a microparticle comprised of an electrically conductive material having a plurality of copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand and being labeled with an electrochemiluminescent moiety;
- (b) incubating said composition to form a complex;
- (c) causing said complex to bind to an assay-ligand immobilized on an electrode;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface and a plurality of electrochemiluminescent moieties immobilized on its surface;
- (iii) an assay-ligand immobilized on an electrode;
- (b) incubating said composition to form a complex;
- and
- (c) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) a microparticle comprised of an electrically conductive material having a plurality of copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand and being labeled with an electrochemiluminescent moiety;
- (iii) an assay-ligand immobilized on an electrode;
- (b) incubating said composition to form a complex;
- and
- (c) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) an assay-ligand immobilized on an electrode;
- (b) incubating said composition to form a complex;
- and
- (c) causing said complex to bind to a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface and a plurality of electrochemiluminescent moieties immobilized on its surface;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample based upon measurements of electrochemiluminescence at an electrode comprising the steps:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) an assay-ligand immobilized on an electrode;
- (b) incubating said composition to form a complex;
- and
- (c) causing said complex to bind to a microparticle comprised of an electrically conductive material having a plurality of copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand and being labeled with an electrochemiluminescent moiety;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample comprising the steps of:
- (a) forming a composition comprising
- (i) said sample;
- and
- (ii) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with the assay-ligand recited in step (c);
- (b) incubating said composition to form a complex;
- (c) causing said complex to bind to an assay-ligand immobilized on an electrode;
- and
- (d) conducting an electrochemiluminescence measurement in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample comprising the steps of:
- (a) forming a composition comprising
- (i) said sample;
- and
- (ii) an assay-ligand immobilized on an electrode;
- (b) incubating said system to form a complex;
- (c) causing said complex to bind to a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample comprising the steps of:
- (a) forming a system comprising
- (i) said sample;
- and
- (ii) an assay-ligand immobilized on an electrode;
- (b) incubating said system to form a complex;
- (c) causing said complex to bind to a microparticle having one or more copies of an assay-ligand immobilized on its surface, said assay-ligand being capable of binding with said analyte or with an assay-ligand;
- and
- (d) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample comprising the steps of:
- (a) forming a composition comprising
- (i) said sample;
- (ii) a microparticle comprised of an electrically conductive material;
- and
- (iii) an assay-ligand immobilized on an electrode;
- (b) incubating said composition to form a complex;
- and
- (c) conducting an electrochemiluminescence measurement in the presence of electrochemiluminescence reactants.
A method for performing an electrochemiluminescence binding assay for an analyte-of-interest present in a sample comprising the steps of:
- (a) forming a composition comprising
- (i) said sample;
- (ii) a microparticle comprised of an electrically conductive material, said microparticle having one or more copies of an assay-ligand and a plurality of electrochemiluminescent moieties immobilized on its surface;
- and
- (iii) an assay-ligand immobilized on an electrode;
- (b) incubating said composition to form a complex;
- and
- (c) conducting an electrochemiluminescence measurement in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand and plurality of electrochemiluminescent moieties immobilized on its surface;
- and
- (ii) an assay-ligand immobilized on an electrode;
- and
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) a microparticle comprised of an electrically conductive material having one or more copies of an assay-ligand immobilized on its surface;
- and
- (ii) an assay-ligand immobilized on an electrode;
- and
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
A method for conducting electrochemiluminescence measurements for a binding analyte-of-interest comprising the steps of:
- (a) forming a complex comprising
- (i) a microparticle comprised of an electrically conductive material, said mircroparticle having one or more copies of an assay-ligand and a plurality of electrochemiluminescent moieties immobilized on its surface;
- and
- (ii) an assay-ligand immobilized on an electrode;
- and
- (b) conducting an electrochemiluminescence measurement at said electrode in the presence of electrochemiluminescence reactants.
Dependencies are inferred from the claim text.