Purification of functionalized fluorescent nanocrystals
AssigneeBio-Pixels Ltd.
InventorsEmilio Barbera-Guillem, Stephanie L. Castro
Life of this patent
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Abstract
Provided are methods for purifying functionalized fluorescent nanocrystals having affinity ligand operably bound thereto by using a solid support matrix in a reactor through which solutions are circulated, and by using an immobilized solid phase formed by operably binding functionalized fluorescent nanocrystals to the solid support matrix. Also provided are purified functionalized fluorescent nanocrystals having affinity ligand operably bound thereto which are purified using the methods according to the present invention to be substantially free of free affinity ligand.
Claims34
A process for purifying functionalized fluorescent nanocrystals having affinity ligand operably bound thereto by using a solid support matrix in a reactor through which solutions are circulated, wherein an immobilized solid phase is formed by operably binding functionalized fluorescent nanocrystals to the solid support matrix, wherein the solid phase is immobilized in the reactor, and wherein the solutions are flowed through the immobilized solid phase to purify the functionalized fluorescent nanocrystals having affinity ligand operably bound thereto.
A method for purifying functionalized fluorescent nanocrystals having affinity ligand operably bound thereto, the method comprising:
- (a) contacting functionalized fluorescent nanocrystals with affinity ligand under suitable conditions for the functionalized fluorescent nanocrystals to become operably bound to affinity ligand in forming a mixture comprising functionalized fluorescent nanocrystals having affinity ligand operably bound thereto, and free affinity ligand;
- (b) contacting the mixture with solid support matrix under suitable conditions for the solid support matrix to become operably bound to functionalized fluorescent nanocrystals having affinity ligand operably bound thereto in forming an immobilized solid phase;
- (c) flowing a solution through the immobilized solid phase in a step of removing free affinity ligand from the solid phase;
- (d) detaching the functionalized fluorescent nanocrystals, comprising functionalized fluorescent nanocrystals having affinity ligand operably bound thereto, from the solid support matrix;
- and
- (e) flowing a solution through the solid support matrix to remove from the solid support matrix the detached functionalized fluorescent nanocrystals having affinity ligand operably bound thereto in yielding purified functionalized fluorescent nanocrystals having affinity ligand operably bound thereto.
A method for purifying functionalized fluorescent nanocrystals having affinity ligand operably bound thereto, the method comprising:
- (a) contacting functionalized fluorescent nanocrystals with solid support matrix under suitable conditions for the functionalized fluorescent nanocrystals to become operably bound to solid support matrix in forming an immobilized solid phase;
- (b) contacting the solid phase with a solution comprising affinity ligand under suitable conditions for affinity ligand to become operably bound to functionalized fluorescent nanocrystals of the solid phase;
- (c) flowing a solution through the solid phase in removing free affinity ligand from the solid phase;
- (d) detaching the functionalized fluorescent nanocrystals, comprising functionalized fluorescent nanocrystals having affinity ligand operably bound thereto, from the solid support matrix;
- and
- (e) flowing a solution through the solid support matrix to remove from the solid support matrix the detached functionalized fluorescent nanocrystals having affinity ligand operably bound thereto in yielding purified functionalized fluorescent nanocrystals having affinity ligand operably bound thereto.
Dependencies are inferred from the claim text.