Growth-promoting agent
AssigneeGroPep Limited
InventorsFrancis John Ballard, Geoffrey Leonard Francis, Geoffrey Owen Regester, Leanna Christine Read, David Andrew Belford
Life of this patent
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Abstract
The invention relates to pharmaceutical or veterinary compositions for the treatment of surface wounds; pharmaceutical or veterinary compositions for the treatment of gastrointestinal injuries, diseases or ulcers; methods of treating surface wounds in animals, including humans; and methods for the treatment of gastrointestinal injuries, diseases or ulcers which compositions and methods include compositions of milk product extracts including growth factors with basic to approximately neutral isoelectric points.
Claims31
A method of treating a surface wound comprising:
- administering to a subject an effective amount of a pharmaceutical or veterinary composition, the composition comprising:
- a milk product extract comprising growth factors from a milk product of an ungulate mammal having a basic to approximately neutral isoelectric point, being enriched in growth promoting activity, and stimulating proliferation of rat myoblasts, the milk protein mixture comprising less than about 20 wt-% of milk protein present in the milk product;
- and a pharmaceutically or veterinarily acceptable diluent, carrier or excipient.
A milk product extract comprising growth factors from a milk product of an ungulate mammal having basic to approximately neutral isoelectric points, being enriched in growth promoting activity, and stimulating proliferation of rat myoblasts, the extract comprising less than about 20 wt-% of milk protein present in the milk product.
A milk product extract prepared by treating a milk product sequentially by:
- clarifying the milk product to provide a milk product with reduced insoluble materials;
- adjusting the pH of the clarified milk product to between about 6.5 and about 8.0;
- contacting the clarified milk product with a cation-exchange resin so that a plurality of cell growth stimulating factors present in the milk product are selectively adsorbed to the cation-exchange resin and wherein the major proteins with acidic isoelectric points in the milk product are not adsorbed;
- eluting the cation-exchange resin with a buffer at high ionic strength or high pH;
- and
- removing salt from the eluate by subjecting the eluate to a concentration step and a diafiltration step.
Dependencies are inferred from the claim text.