Non-obligate predatory bacterium burkholderia casidaeand uses thereof
AssigneesVirginia Tech Intellectual Properties, Inc., Virginia Polytechnic Institute and State University, The Penn State Research Foundation
InventorsLester Earl Casida, Jr., Joseph Oliver Falkinham, III, Cody Christopher Cain
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Abstract
A novel predator bacterium Burkholderia casidae is disclosed. The invention is directed to the isolation and use of Burkholderia casidae to control microbial diseases of plants. The genetic, biochemical and physiological characteristics of Burkholderia casidae are described. Biocontrol compositions comprising Burkholderia casidae, and antimicrobial compounds and antimicrobial preparations prepared from Burkholderia casidae are also disclosed, as are methods for accomplishing all of the foregoing.
Claims34
A substantially pure culture or suspension of a bacterium Burkholderia casidae or variant thereof, which Burkholderia casidae or variant exhibits biocontrol activity against a microorganism, and has
- a) a 16S rRNA gene comprising a sequence that is at least 97% similar to the sequence of SEQ ID NO:1 as determined by Clustal Analysis;
- and
- b) a cellular fatty acid composition comprising about 16% to about 20% C16:0 fatty acid, about 18% to about 22% C16:1 fatty acid, and about 35% to about 45% C18:1 (11,12) fatty acid;
- wherein the microorganism is a bacterium, yeast, filamentous fungi, protozoan or algae.
A substantially pure culture or suspension of Burkholderia casidae strain 2.2N having the accession number ATCC 55961, or a variant thereof.
An antimicrobial preparation comprising an alcohol-extract of a cell, cyst, culture, suspension, cell-free filtrate or cell fraction of a bacterium Burkholderia casidae or variant thereof, which Burkholderia casidae or variant exhibits biocontrol activity against a microorganism, and has
- a) a 16S rRNA gene comprising a sequence that is at least 97% similar to the sequence of SEQ ID NO:1 as determined by Clustal Analysis;
- and
- b) a cellular fatty acid composition comprising about 16% to about 20% C16:0 fatty acid, about 18% to about 22% C16:1 fatty acid, and about 35% to about 45% C18:1 (11,12) fatty acid;
- wherein the microorganism is a bacterium, yeast, filamentous fungi, protozoan or algae.
A method for producing an antimicrobial preparation from Burkholderia casidae or variant thereof, comprising:
- a) boiling an alcoholic mixture comprising a cell, culture, suspension, cell-free filtrate or cell fraction of Burkholderia casidae or variant thereof, and an alcohol;
- b) clarifying the boiled mixture;
- c) mixing the boiled mixture with magnesium silicate;
- d) collecting the magnesium silicate;
- e) washing the magnesium silicate with water;
- and
- f) eluting antifungal compounds from the magnesium silicate with an alcoholic solution, thereby producing the antimicrobial preparation.
A method for isolating a Burkholderia casidae or variant thereof, which comprises
- (a) determining the 16S rRNA sequence and the cellular fatty acid composition of a predator bacterium which exhibits biocontrol activity against a microorganism;
- and
- (b) identifying a bacterium having (i) a 16S rRNA gene comprising a sequence that is at least 97% similar to the sequence of SEQ ID NO:1 as determined by Clustal Analysis, and (ii) a cellular fatty acid composition comprising about 16% to about 20% C16:0 fatty acid, about 18% to about 22% C16:1 fatty acid, and about 35% to about 45% C 18:1 (11,12) fatty acid;
- thereby isolating the Burkholderia casidae or variant thereof.
Dependencies are inferred from the claim text.