Contrast medium for near infrared diagnosis
AssigneeInstitut fur Diagnostikforschung GmbH an der Freien Universitat Berlin
InventorsKai Licha, Björn Riefke, Werner Weitschiess, Dieter Heldmann, Violetta Sudmann
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Abstract
This invention relates to colloidal systems charged with polymethine dyes and having suitable photophysical and pharmacological properties, their use as a contrast medium in fluorescence and transillumination diagnostics in the near infrared spectral range, as well as methods for their production.
Claims11
An in vivo contrast medium and target tissue combination, wherein said target tissue is selected from the group consisting of inflamed tissue and tumor tissue, said in vivo contrast medium comprising a colloidal system with particles having a size range from 5 nm to 10 μm, said colloidal system further comprising at least one dye fluorescing in the wavelength range from 600 nm to 1200 nm upon direct absorption of monochromatic light in the wavelength range from 600 nm to 1200 nm, said at least one dye is selected from the group consisting of the general formula I,
- wherein
- R1-R10 represent the residue E1, where E1 is a hydrogen,
- wherein
- Q is one of the following fragments:
- wherein
- b=0,
- R11 represents a hydrogen atom,
- R12 represents a hydrogen atom or a saturated or unsaturated, branched or non-branched C1-C50 alkyl chain, and said chain or parts thereof form a cyclic C5-C6, or a bicyclic C10 unit interrupted and/or replaced by oxygen atoms, sulfur atoms, nitrogen atoms, carboxylic acid ester, carboxylic acid amide, urea, thiourea, carbamate, ether groupings, or represent a hydroxypolyoxyethylene or methoxypolyoxyethylene chain or a branched or non-branched C1-C10 alkyl chain substituted with 1 to 19 fluorine atoms,
- where X and Y are both
- fragment,
- and wherein
- R13 and R14 independently represent hydrogen.
A method for in vivo near infrared diagnosis of target tissue in an organism using an in vivo contrast medium capable of absorption and/or fluorescence in the 600 nm to 1200 nm wavelength range, said target tissue is selected from the group consisting of inflamed tissue and tumor tissue, said method comprising the steps of:
- (a) preparing an in vivo contrast medium comprising a colloidal system, said colloidal system having a particle size range from 5 nm to 10 μm, said colloidal system comprising at least one dye, said at least one dye absorbs and/or fluoresces in the wavelength range from 600 nm to 1200 nm, said in vivo contrast medium being amenable to accumulation in said target tissue, said in vivo contrast medium being amenable to degradation by liver tissue at a faster rate than degradation by said target tissue;
- (b) performing intravenous administration of said in vivo contrast medium into an organism;
- (c) sufficiently irradiating said organism with monochrome light of a wavelength range of about 600 nm to 1200 nm to cause said in vivo contrast medium to fluoresce;
- and
- (d) scanning said irradiated organism using a CCD camera to obtain images of said in vivo contrast medium to detect the diseased tissue.
An in vivo contrast medium and target tissue combination, wherein said target tissue is selected from the group consisting of inflamed tissue and tumor tissue, said in vivo contrast medium comprising a colloidal system, said colloidal system having a particle size range from 5 nm to 10 μm, said colloidal system comprising a dye, said dye absorbing and/or fluorescing in the wavelength range from 600 nm to 1200 nm, said dye is 1,1′,3,3,3′,3′-hexamethyl indotricarbocyanine iodide of Example 1.
A method for imaging tumor tissue comprising the steps of:
- (a) performing intravenous administration of an in vivo contrast medium into a body suspected of harboring tumor tissue, said in vivo contrast medium comprising colloidal system with a particle size range from 5 nm to 10 μm, said colloidal system comprising one or more dyes, said one or more dyes absorbing and/or fluorescing in the wavelength range from 600 nm to 1200 nm, said in vivo contrast medium being amenable to accumulation in said tumor tissue, said in vivo contrast medium being amenable to degradation by liver tissue at a faster rate than degradation by said tumor tissue;
- (b) irradiating said body with monochrome light in the wavelength range of about 600 nm to 1200 nm to cause said in vivo contrast medium of step (b) to absorb and/or fluoresce;
- and
- (c) scanning said irradiated body to yield images of said tumor tissue in said body.
Dependencies are inferred from the claim text.