Use of an earlier known separation material and novel forms of the material
AssigneeAmersham Pharmacia Biotech AB
InventorsEva Berggren, Ilya Zelikman
Life of this patent
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Abstract
The use of a gel produced by polymerization of a dextran derivative which exhibits groups that contain an alkene structure, for separating nucleic acid by electrophoresis.
Claims16
In a method for separating nucleic acids by the electrophoresis, wherein a gel is utilized as an electrophoresis medium and an electric field is applied across the surface of the gel to separate the nucleic acids, the improvement comprising using, as the gel, a gel prepared by polymerization of a dextran derivative having groups that contain an alkene structure.
A gel produced by polymerization of a dextran derivative exhibiting groups having an alkene structure comprising:
- H2C═CR—B—
- where
- R is H, a hydrocarbon group, F, Cl, Br or CN, and
- the free valance binds to an hydroxylic oxygen in dextran, and
- B is a straight, branched or cyclic hydrocarbon bridge which is optionally broken by ether oxygen and/or substituted with one or more alcoholic hydroxyl groups
- said gel being hydrated with a buffer giving a pH value in the range of 10-13.
A gel produced by polymerization of a dextran derivative exhibiting groups having an alkene structure comprising:
- H2C═CR—B—
- where
- R is H, a hydrocarbon group, F, Cl, Br or CN, and
- the free valance binds to an hydroxylic oxygen in dextran, and
- B is a straight, branched or cyclic hydrocarbon bridge which is optionally broken by ether oxygen and/or substituted with one or more alcoholic hydroxyl groups
- said gel being hydrated with a buffer set to a pH value in the range of 7-10 and containing a denaturing agent which dissociates double-stranded nucleic acid.
Dependencies are inferred from the claim text.