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AssigneeThe Regents of the University of California
InventorsJ. Courtney Davidson, Joseph W. Balch
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A sample injection method for horizontal configured multiple chromatography or electrophoresis units, each containing a number of separation/analysis channels, that enables efficient introduction of analyte samples. This method for loading when taken in conjunction with horizontal microchannels allows much reduced sample volumes and a means of sample stacking to greatly reduce the concentration of the sample. This reduction in the amount of sample can lead to great cost savings in sample preparation, particularly in massively parallel applications such as DNA sequencing. The essence of this method is in preparation of the input of the separation channel, the physical sample introduction, and subsequent removal of excess material. By this method, sample volumes of 100 nanoliter to 2 microliters have been used successfully, compared to the typical 5 microliters of sample required by the prior separation/analysis method.
In a method of electrokinetic sample injection in chromatography and electrophoresis analysis using a microchannel apparatus comprising at least one microchannel and at least one sample load well, the improvement including:
A modified electrokinetic sample injection method for use in chromatography and electrophoresis analysis using a microchannel apparatus having at least one microchannel intermediate an input opening and an output opening, and having an input reservoir and an output reservoir located adjacent the input and output openings, including:
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