Method for producing nitrile hydratase
AssigneeThe Institute of Physical and Chemical Research
InventorsIsao Endo, Masafumi Yohda, Masafumi Odaka, Masaki Nojiri
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Abstract
There is disclosed a method for producing nitrile hydratase comprising culturing a transformant cell which is obtained by transforming a host cell of bacterium not belonging to the genus Rhodococcus with a vector (1) which contains a nucleotide sequence encoding at least the α a chain and the β chain of nitrile hydratase, or the vector (1) and a vector (2) which contains a nucleotide sequence encoding at least the β chain of the nitrile hydratase, and collecting produced nitrile hydratase, wherein the culture of the transformant cell is performed at a temperature of 35° C. or less.
Claims5
A method for producing nitrile hydratase comprising culturing a transformant cell which is obtained by
- transforming an E. coli cell with a vector (1) which contains a nucleotide sequence encoding at least the α subunit and β subunit of nitrile hydratase of Rhodococcus N-771, and a nucleotide sequence encoding an activator for the nitrile hydratase;
- and
- collecting produced nitrile hydratase,
- wherein the culture of the transformant cell is performed at a temperature of about 27° C.
A method for producing nitrile hydratase comprising culturing a transformant cell which is obtained by
- transforming an E. coli cell with a vector (1) which contains a nucleotide sequence encoding at least the α subunit and β subunit of nitrile hydratase of Rhodococcus N-771, and a nucleotide sequence encoding an activator for the nitrile hydratase and a vector (2) which contains a nucleotide sequence encoding at least the β subunit of the nitrile hydratase but not the α subunit;
- and
- collecting produced nitrile hydratase,
- wherein the culture of the transformant cell is performed at a temperature of about 35° C. or less.
Dependencies are inferred from the claim text.