Methods for the early diagnosis of ovarian, breast and lung cancer
AssigneeBoard of Trustees of the University of Arkansas
InventorTimothy J. O'Brien
Life of this patent
Estimating the term…
Abstract
The disclosed nucleic acid primer sets, used in combination with quantitative amplification (PCR) of tissue cDNA, can indicate the presence of specific proteases in a tissue sample. The detected proteases are themselves specifically overexpressed in certain cancers, and their presence may serve for early detection of associated ovarian and other malignancies, and for the design of interactive therapies for cancer treatment.
Claims9
A method of diagnosing ovarian, breast or lung cancer in an individual, comprising the steps of:
- (a) obtaining a biological sample from an individual;
- and
- (b) detecting PUMP-1 in said sample, wherein over-expression of PUMP-1 in said sample compared to a sample of normal tissue is indicative of the presence of ovarian, breast or lung cancer in said individual.
A method for detecting malignant ovarian, breast or lung hyperplasia in a biological sample, comprising the steps of:
- (a) isolating protein from said sample;
- and
- (b) detecting PUMP-1 protein in said sample, wherein over-expression of said PUMP-1 protein in said sample compared to a sample of normal tissue is indicative of the presence of malignant ovarian, breast or lung hyperplasia.
A method for detecting ovarian malignant hyperplasia in a biological sample, comprising the steps of:
- (a) isolating proteases present in said biological sample;
- and
- (b) detecting specific proteases selected from the group consisting of hepsin, SCCE, and PUMP-1 present in said biological sample, wherein over-expression of said proteases in said sample compared to normal ovarian tissue sample is indicative of the presence of malignant ovarian hyperplasia.
Dependencies are inferred from the claim text.