Methods for determining isolated p27 protein levels and uses thereof
AssigneesMemorial Sloan Kettering Cancer Center, Fred Hutchinson Cancer Research Center
InventorsJames M. Roberts, Peggy L. Porter, Kornelia Polyak, Joan Massague, Andrew Koff
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Abstract
The subject invention is directed to the discovery of a protein involved in regulation of cell-cycle progression, and includes reagents and methods related thereto.
Claims13
A method for determining the relative amount of p27 protein in a cell or sample of cells (cell sample) comprising (i) determining the level of a p27 protein having a sequence of one of SEQ ID Nos. 2, 4, and 6 in the cell sample, and (ii) comparing the level of the p27 protein in the cell sample with the level of a wild-type p27 protein determined for a control cell, wherein the wild-type p27 protein inhibits activation of a cyclin E-Cdk2 (cyclin-dependent kinase-2) complex and has an amino acid sequence of one of SEQ ID Nos. 2, 4, and 6.
A method for diagnosing a hyperproliferative disorder characterized by abnormal cell proliferation in a patient, comprising:
- (i) ascertaining the level of a p27 protein having an amino acid sequence of one of SEQ ID Nos. 2, 4, and 6 in a sample of cells from the patient;
- and (ii) diagnosing the presence or absence of a hyperproliferative disorder including utilizing the ascertained level of the p27 protein, wherein a reduced level of the p27 protein in the sample, relative to a level of a wild-type p27 protein in a control sample of normal cells, correlates with the presence of a hyperproliferative disorder, and the wild-type p27 protein inhibits activation of a cyclin E-Cdk2 (cyclin-dependent kinase) complex and has an amino acid sequence of one of SEQ ID Nos. 2, 4, and 6.
A method for evaluating a cancer patient's prognosis, comprising (i) ascertaining the level of a p27 protein having an amino acid sequence of one of SEO ID Nos. 2, 4, and 6 in a sample of cancer cells from the patient;
- and (ii) comparing the ascertained level of the p27 protein to a level of wild-type p27 protein in a normal control sample of cells, wherein a reduced level of the p27 protein in the sample correlates with an increased risk of recurrence of a cancer, and wherein the wild-type p27 protein inhibits activation of a cyclin E-Cdk2 (cyclin-dependent kinase-2) complex and has an amino acid sequence of one of SEQ ID Nos. 2, 4, and 6.
A method for determining the relative amount of p27 protein in a cell or sample of transformed cells (cell sample) from a patient comprising (i) determining the level of p27 protein having an amino acid sequence of one of SEQ ID Nos. 2, 4, and 6 in the cell sample, and (ii) comparing the level of p27 protein in the cell sample with the level of p27 protein determined for a control cell, wherein the p27 protein inhibits activation of a cyclin E-Cdk2 (cyclin-dependent kinase) complex and has an amino acid sequence of one of SEQ ID Nos. 2, 4, and 6.
Dependencies are inferred from the claim text.