Polynucleotides encoding choline monooxygenase and plants transformed therewith
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsAndrew D. Hanson, Bala Rathinasabapathi, Michael Burnet
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Abstract
A full length choline monooxygenase (CMO) cDNA was cloned from spinach and used to transform plants which do not naturally express CMO. A method is presented to improve stress tolerance of crops following engineering of CMO and BADH in plants that lack glycine betaine accumulation. Also provided are fragments useful as probes to isolate other CMO-type genes, and antisense sequences which inhibit the production of CMO. Reduction of glycine betaine as a consequence of antisense expression of CMO in species naturally accumulating glycine betaine, improves the transgenic plant's tolerance toward pathogens and pests and/or enhances its nutritional quality.
Claims48
An isolated polynucleotide molecule that encodes a protein exhibiting choline monooxygenase (CMO) activity, said polynucleotide molecule being capable of hybridizing to a polynucleotide comprising the sequence of SEQ ID NO:
- 1 under high stringency conditions.
An isolated polynucleotide molecule that encodes a protein exhibiting choline monotygenase (CMO) sequence of SEQ ID NO:
- 3 activity, said polynucleotide molecule being capable of hybridizing to a polynucleotide having the sequence of SEQ ID NO:
- 3 under high stringency conditions.
Dependencies are inferred from the claim text.