Translation enhancer element of the human amyloid precursor protein gene
AssigneeThe Brigham and Women's Hospital, Inc.
InventorJack Rogers
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Abstract
The present invention is directed to a DNA element that enhances the translation of the human amyloid precursor protein (APP) gene. The enhancer may be incorporated into expression vectors to enhance recombinant protein production. In addition, the invention is directed to an assay that utilizes vectors containing the translation enhancer element for the purpose of identifying agents that modulate the expression of the human amyloid precursor protein. These agents will ultimately be used to suppress APP expression in patients with Alzheimer's disease.
Claims10
A substantially pure DNA molecule comprising:
- a) a translation enhancer element consisting essentially of the nucleotide sequence of SEQ ID NO:1;
- b) a non-homologous gene operably linked to said translation enhancer element.
A vector for recombinantly expressing a peptide or protein in a eukaryotic cell comprising:
- a) a promoter which is active in said eukaryotic cell;
- b) a translation enhancer element consisting of the nucleotide sequence of SEQ ID NO:1, wherein said element is 3′ to said promoter;
- c) a DNA sequence encoding said peptide or protein wherein said DNA sequence:
- i) lies 3′ to said translation enhancer element;
- ii) is operably linked to said promoter;
- and
- iii) is non-homologous to said translation enhancer element.
Dependencies are inferred from the claim text.