Nucleotides and analogs having photoremoveable protecting groups
AssigneeAffymetrix, Inc.
InventorsStephen P. A. Fodor, Lubert Stryer, J. Leighton Read, Michael C. Pirrung
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Abstract
A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.
Claims17
A compound having a photoremoveable protecting group covalently attached to a C-3′ or C-5′ oxygen atom, wherein said compound is selected from the group consisting of natural or unnatural nucleoside phosphoramidites and natural or unnatural nucleoside H-phosphonates.
A method of activating a terminal nucleotide in a nucleic acid, said terminal nucleotide having a photoremoveable protecting group, said method comprising removing said photoremoveable protecting group from a latent C5′ hydroxy moiety in said nucleic acid.
Dependencies are inferred from the claim text.