Polypeptides endowed with a larvicidal activity toward Lepidoptera
AssigneeInstitut Pasteur and Institute National de la Rocherche Agronomic
InventorsVincent Sanchis, Didier Lereclus, Ghislaine Menou, Marguerite-Marie Lecadet, Daniel Martouret, Raymond Dedonder
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Abstract
This invention relates to plant cells, plants, and seeds expressing a polypeptide having larvicidal activity. In particular, the invention relates to plant cells, plants, and seeds expressing the N-terminal region of a polypeptide toxic against the larvae of Lepidoptera of the Noctuidae family, and preferably against S.littoralis.
Claims27
A larvicidal composition comprising a polypeptide with larvicidal activity toward lepidoptera having the amino acid sequence of SEQ ID NO:2 or larvicidal fragments thereof.
A larvicidal composition comprising a polypeptide with larvicidal activity toward lepidoptera, wherein said polypeptide is expressed by a nucleotide sequence coding for at least part of the N-terminal region of a polypeptide specifically toxic towards larvae of Lepidoptera of the family of Noctuidae, said nucleotide sequence being capable of hybridizing with a gene at 42° C. in a solution containing 5×SSC, 30% formamide, and 1×Denhardt, wherein said gene expresses a polypeptide having the amino acid sequence of SEQ ID NO:2 or larvicidal fragments thereof.
A polypeptide having larvicidal activity against S. littoralis and which forms a specific immunological complex with an antibody directed against a polypeptide comprising amino acids 1-250 of SEQ ID NO:2 or the fall length of SEQ ID NO:2.
A composition having larvicidal activity against S. littoralis, which composition comprises a carrier and larvicidally effective amount of a polypeptide having larvicidal activity against S. littoralis and which forms a specific immunological complex with an antibody directed against a polypeptide comprising amino acids 1-250 of SEQ ID NO:2 or the full length of SEQ ID NO:2.
Dependencies are inferred from the claim text.