Nano-grid micro reactor and methods

A method for organizing items, the method comprising:

• placing items that each have at least one chemical or biological component associated therewith into a plurality of holding locations within a holding member such that the items are disposed below a top surface of the holding member;
• enclosing the holding member within a housing containing a membrane that is spaced apart from the holding member;
• and
• forcing the membrane onto the top surface of the holding member to cover the holding locations and to provide seal between the holding locations.

A method for performing assays, the method comprising:

• placing solid supports and/or cells having at least one chemical or biological component associated therewith into a plurality of holding locations within a holding member such that the solid supports and/or cells are disposed below a top surface of the holding member;
• placing the holding member into a housing that is configured to permit the transmission of light;
• forcing a membrane onto the top surface of the holding member to cover the holding locations and to provide seal between the holding locations, with the membrane being spaced apart from the solid supports or cells;
• and
• evaluating any chemical or biological reactions occurring within the holding locations through the housing.

A method for performing assays, the method comprising:

• placing solid su ports that each have at least one chemical synthesized thereon into a plurality of holding loans within a holding member such that the solid supports are disposed below a top surface of the holding member;
• placing the holding member into a housing that is configured to permit the transmission of light;
• forcing a membane having an organism attached thereto onto the top surface of the holding member to cover the holding locations with the organism and to provide seal between the holding locations, with the organism being spaced apart from the solid supports;
• releasing at least a portion of the chemicals from the solid supports by directing radiation through the housing;
• and
• evaluating any chemical reactions resulting from an interaction of the released chemicals with the organism.