Human patched genes and proteins, and uses related thereto
AssigneeCuris, Inc.
InventorDavid H. Bumcrot
Life of this patent
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Abstract
The present invention relates to the discovery of a new member of the hedgehog receptor family, referred to herein as human ptc-2 (for patched-2 protein). The human ptc-2 polypeptides of the present invention include polypeptides which bind the products of the hedgehog gene family. Hedgehog family members are known for their broad involvement in the formation and maintenance of ordered spatial arrangements of differentiated tissues in vertebrates, both adult and embryonic, and can be used to generate and/or maintain an array of different vertebrate tissue both in vitro and in vivo.
Claims13
An isolated nucleic acid comprising a coding sequence for a human patched-2 (ptc-2) polypeptide at least 90% identical to SEQ ID No. 2, which ptc-2 polypeptide binds a naturally occurring hedgehog protein.
An isolated nucleic acid comprising a coding sequence for a polypeptide comprising a patched-2 (ptc-2) amino acid sequence that binds a naturally occurring hedgehog protein, which coding sequence hybridizes under stringent conditions including a wash step of 0.2×SSC at 50° C. to the sequence of SEQ ID. No. 1.
A nucleic acid comprising (i) a coding sequence for a polypeptide comprising a patched-2 (ptc-2) amino acid sequence that binds a naturally occurring hedgehog polypeptide, which coding sequence hybridizes under stringent conditions including a wash step of 0.2×SSC at 50 ° C. to the sequence of SEQ ID. No. 1;
- and (ii) a heterologous transcriptional regulatory sequence operably linked thereto.
An oligonucleotide probe comprising a nucleotide sequence which hybridizes under stringent conditions including a wash step of 0.2×SSC at 50° C. to a naturally occurring human patched-2 (ptc-2) gene having the sequence of SEQ ID No. 1, but not to a naturally occurring human patched-1 (ptc-1) gene.
A nucleic acid comprising (i) a coding sequence for a human patched-2 (ptc-2) polypeptide at least 95% identical to SEQ ID No. 2, which ptc-2 polypeptide binds a naturally occurring hedgehog protein and (ii) a heterologous transcriptional regulatory sequence operably linked thereto.
Dependencies are inferred from the claim text.