Neuron promoter and uses
AssigneeMcGill University
InventorsFreda Diane Miller, Andrew Gloster, Carrie Grace Causing, Jean George Toma
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Abstract
The present invention relates to a DNA expression vector comprising a Tαl α-tubulin promoter operatively linked upstream to a marker gene sequence relative to the direction of transcription in a eukaryotic expression vector adapted to be introduced into a mammal, or ancestor of the mammal at an embryonic stage and the promoter is controlling transcription of the marker gene. The present invention also relates to a method for testing material suspected of promoting neurogenesis and neuronal growth which comprises exposing a transgenic mammal according to the present invention to the material and detecting the expressed marker gene as an indication of neurogenesis and neuronal growth.
Claims4
An isolated nucleic acid molecule comprising a Tαl α-tubulin promoter, wherein said promoter comprising the 5′ flanking region immediately upstream of the 5′ untranslated region of a gene encoding rat Tαl α-tubulin, wherein said 5′ flanking region comprises an SP-2 site, a consensus PEA-3 site, an NF-IL-6 binding site, a γ-interferon response element and a consensus sequence binding element for the CAATT-enhancer binding protein.
A DNA expression vector comprising a Tαl α-tubulin promoter, wherein said promoter comprising the 5′ flanking region immediately upstream of the 5′ untranslated region of a gene encoding rat Tαl α-tubulin, wherein said 5′ flanking region comprises an SP-2 site, a consensus PEA-3 site, an NF-IL-6 binding site, a γ-interferon response element and a consensus sequence binding element for the CAATT-enhancer binding protein.
Dependencies are inferred from the claim text.