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US 6,309,869 B1Granted
Polypeptides having acid phosphatase activity and nucleic acids encoding same
AssigneeNovozymes Biotech, Inc.
InventorsDebbie S. Yaver, Randy M. Berka, Michael W. Rey
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Abstract
The present invention relates to isolated polypeptides having acid phosphatase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Claims13
An isolated polypeptide having acid phosphatase activity, selected from the group consisting of:
- (a) a polypeptide having an amino acid sequence which has at least 80% identity with amino acids 19 to 318 for the mature polypeptide of SEQ ID NO. 2;
- (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under medium stringency conditions with (i) nucleotides 138 to 1252 of SEQ ID NO. 1, (ii) the cDNA sequence contained in nucleotides 138 to 1252 of SEQ ID NO. 1, or (iii) a complementary strand of (i) or (ii), wherein medium stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 35% formamide, and washes at 55° C. for 15 minutes in 2×SSC, 0.2% SDS;
- and
- (c) a fragment of (a) or (b), that has acid phosphatase activity.
1 more element
Dependencies are inferred from the claim text.