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US 6,309,862 B1Granted
Methionine containing animal cell culture medium and its use
AssigneeBionative AB
InventorsMats Jarekrans, Hans Olovsson
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Abstract
A nutrient medium for protein producing cells, characterized in that said medium consists essentially of the following components: a physiological saline containing Ca2+, K+, Mg2+ and Na+, an energy source, a pH buffer and methionine in an amount of 0.015-2.0 g/liter, and optionally antibiotics. The invention further relates to use of the nutrient medium for cells and human leukocytes.
Claims18
A nutrient medium for protein producing cells, wherein said medium consists of the following components:
- an aqueous physiological saline solution containing Ca2+, K+, Mg2+ and Na+, an energy source, a pH buffer, agamma-plasma, and methionine in an amount greater than 0.015 g/liter and not more than 2.0 g/liter.
Dependencies are inferred from the claim text.