Method for removing N-terminal methionine
AssigneeTakeda Chemical Industries, Ltd.
InventorsOsamu Nishimura, Masato Suenaga, Hiroaki Ohmae, Shinji Tsuji
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Abstract
The present invention provides a method for chemically removing a N-terminal methionine residue selectively, specifically and efficiently from a peptide or a salt thereof having an optionally oxidized methinine residue at its N-terminal. The method reacts a peptide or a salt thereof having an optionally oxidized methinine residue at its N-terminal with an α-diketone derivative, followed by hydrolysis.
Claims11
A method for removing an oxidized or unoxidized N-terminal methionine residue from a recombinantly produced polypeptide to form a bioactive polypeptide, comprising reacting a polypeptide, or a salt thereof, having said methionine residue at its N-terminus, with an α-diketone or a salt thereof in the presence of a transition metal ion and a base, and subjecting the obtained product to hydrolysis, and recovering the resultant product to obtain a bioactive polypeptide without its N-terminal methionine residue.
Dependencies are inferred from the claim text.