US 6,309,842 B1 · Use of modified tethers in screening compound libraries

Abstract

The invention provides methods for screening libraries of complexes for compounds having a desired property, especially, the capacity to agonize, bind to, or antagonize a cellular receptor. The complexes in such libraries comprise a compound under test, a tag recording at least one step in synthesis of the compound, and a tether susceptible to modification by a reporter molecule. Modification of the tether is used to signify that a complex contains a compound having a desired property. The tag can be decoded to reveal at least one step in the synthesis of such a compound.

Claims4

Dependent claims

A method of screening compounds for capacity to transduce a signal through a cellular receptor, comprising

providing a plurality of supports, each support bearing multiple copies of a compound under test, and a tether susceptible to modification by a reporter molecule;
contacting the supports with cells having a receptor and a DNA fragment encoding the reporter molecule;
freeing a portion of the multiple copies of each of the compounds under test from the supports, whereby at least one compound transduces a signal through the receptor of a cell causing expression of the reporter molecule, which reporter molecule is released from the cell and modifies the tether of the support from which a portion of the multiple copies of the compound transducing the signal was freed;
and
isolating the support having the modified tether, which support bears the compound transducing the signal.

2 more elements

Dependencies are inferred from the claim text.

Use of modified tethers in screening compound libraries

Life of this patent

Use of modified tethers in screening compound libraries

Life of this patent

Abstract

Claims4