Expression augmenting sequence elements (ease) for eukaryotic expression systems
AssigneeImmunex Corporation
InventorsArvia E. Morris, James N. Thomas
Life of this patent
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Abstract
There are disclosed nucleotide sequences which can improve expression of recombinant proteins two to eight fold in stable cell pools when present in an expression vector.
Claims20
A polynucleotide selected from the group consisting of DNA comprising nucleotides 11303-12192 of SEQ ID NO:1, nucleotides 11538-12165 of SEQ ID NO:1, fragments of the foregoing DNA that have expression augmenting activity, DNA complementary to the foregoing DNA, and combinations of the foregoing DNA that have expression augmenting activity.
A polynucleotide comprising nucleotides 11538-12165 of SEQ ID NO:1.
A method of identifying expression augmenting sequences, the method comprising the steps of identifying HMGI-(Y) binding sites within a sequence, cloning one or more of the sites from the transformed cell line, and determining if at least one cloned site exhibits expression augmenting activity.
Dependencies are inferred from the claim text.