Methods for quantitating the efficacy of oral care products
AssigneeKoninkiijke Philips Electronics N.V.
InventorsLokanathan M. Iyer, Robert E. Akridge, James C. McInnes
Life of this patent
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Abstract
Methods are included for quantitating the efficacy of oral care products at dislodging cells from biofilm test surfaces or inhibit or delay the accumulation of cells on a test surface. More specifically, the inventive methods measure the effectiveness of compositions and appliances in a test environment that models biofilm surface orientations that are encountered on a tooth surface, i.e. interproximal and subgingival tooth surfaces. The use of test surfaces that are removably attachable to a tooth prosthesis allow a variety of different quantitative methods to be applied to determine the amount of cells removed or deposited in a biofilm.
Claims35
A method for quantitating the efficacy of an oral care product in the removal of a biofilm, the method comprising:
- (a) treating a test surface with saliva;
- (b) binding cells to the test surface to form a biofilm test surface;
- (c) removing cells not bound to the biofilm test surface;
- (d) mounting the biofilm test surface in a tooth prosthesis to form a biofilm tooth prosthesis;
- (e) placing the biofilm tooth prosthesis into a typodont;
- (f) placing the typodont into a collection chamber;
- (g) treating the biofilm tooth prosthesis with an oral care product;
- and
- (h) quantitatively determining the amount of cells dislodged from the biofilm test surface, said quantitation of cells indicating the efficacy of said oral care product in biofilm removal.
A method for quantitating the efficacy of an oral care product in the removal of a biofilm, the method comprising:
- (a) treating a test surface with saliva;
- (b) binding cells to the test surface to form a biofilm test surface;
- (c) removing cells not bound to the biofilm test surface;
- (d) mounting the biofilm test surface in a tooth prosthesis to form a biofilm tooth prosthesis;
- (e) placing the biofilm tooth prosthesis into a typodont;
- (f) treating the biofilm tooth prosthesis with an oral care product;
- and
- (g) quantitatively determining the amount of cells in the biofilm after completion of step (f), said guantitation of cells indicating the efficacy of said oral care product in biofilm removal.
A method for measuring the efficacy of an oral care product in preventing or inhibiting the formation of dental plaque, the method comprising:
- (a) treating a test surface with saliva;
- (b) binding cells to the test surface to form a biofilm test surface;
- (c) mounting the biofilm test surface in a tooth prosthesis to form a biofilm tooth prosthesis;
- (d) placing the biofilm tooth prosthesis into a typodont;
- (e) treating the test surface with an oral care product;
- (f) incubating the test surface with cells under conditions conducive to the formation, of a biofilm on the test surface;
- (g) removing cells not bound to the test surface or bound in a biofilm formed on the test surface;
- and
- (h) quantitatively determining the amount of cells in the biofilm, said quantitation of cells indicating the efficacy of said oral care product in biofilm removal.
Dependencies are inferred from the claim text.