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US 6,309,832 B1Granted
Method to detect IgE
AssigneeHeska Corporation
InventorsGlenn R. Frank, James P. Porter, Keith E. Rushlow, Donald L. Wassom
Life of this patent
Estimating the term…
Abstract
The present invention includes a method to detect IgE using a human Fc epsilon receptor (FcεR) to detect IgE antibodies in a biological sample from a cat, a dog, or a horse. The present invention also relates to kits to perform such methods.
Claims20
A method to detect flea allergy dermatitis comprising:
- a. immobilizing a flea allergen on a substrate;
- b. contacting said flea allergen with a putative IgE-containing composition under conditions suitable for formation of an antigen:IgE complex bound to said substrate;
- c. removing non-bound material from said substrate under conditions that retain antigen:IgE complex binding to said substrate;
- and
- d. detecting the presence of said antigen:IgE complex by contacting said antigen:IgE complex with a FcεR molecule and removing non-bound FcεR molecule under conditions that retain bound FcεR molecule, the presence of said FcεR molecule indicating the presence of said IgE:antigen complex.
2 more elements
A kit for detecting flea allergy dermatitis comprising a human Fcε receptor (FcεR) molecule and at least one flea allergen.
A method for the detection of flea allergy dermatitis comprising:
- a. immobilizing on a substrate an FcεR molecule;
- b. contacting said substrate with a putative IgE-containing composition under conditions suitable for formation of complex formation between said IgE and said FcεR molecule, to form an IgE-containing complex;
- and
- c. detecting the presence of said IgE-containing complex by contacting said complex with at least one flea allergen and removing non-bound allergen under conditions that retain bound allergen, the presence of said flea allergen indicating the presence of said IgE-containing complex.
1 more element
Dependencies are inferred from the claim text.