US 6,309,827 B1 · Simultaneous collection of DNA and non-nucleic analytes

Abstract

This invention provides for a rapid and convenient method of simultaneous collection of both genomic and diagnostic information from a single sample on a bibulous pad by differential extraction of the diagnostic information from the genomic information. It is a surprising discovery of this invention that a PCR assay on the contents of the bibulous pad provides results comparable in reliability, specificity, and sensitivity to the best available serum (blood) based assays. The assays of this invention can be used to confirm each other, either by detecting the genomic information leading to the diagnostic information, or by detecting in the genomic information, a predisposition to a disease and confirming the presence of the disease through diagnostic testing.

Claims22

Dependent claims

A method for obtaining and detecting a genomic marker and an extracellular analyte from a single collection step, said method comprising:

(i) collecting a sample onto a bibulous solid support having a fibrous matrix, said sample comprising both (a) whole cells containing a genomic marker and (b) an extracellular analyte;
(ii) selectively obtaining extracellular analyte under conditions in which the genomic marker remains in cells within the fibrous matrix of the solid support;
(iii) obtaining genomic marker from the solid support by lysing the whole cells and freeing genomic marker from the fibrous matrix of the solid support;
and,
(iv) detecting the analyte and genomic marker.

2 more elements

Dependencies are inferred from the claim text.

Simultaneous collection of DNA and non-nucleic analytes

Life of this patent

Simultaneous collection of DNA and non-nucleic analytes

Life of this patent

Abstract

Claims22