Composition and method for preparation, storage and activation of large populations of immotile sperm
AssigneeUniversity of Kansas Medical Center
InventorsJoseph S. Tash, Gerácimo E. Bracho
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Abstract
A method and composition for extending the viability of immotile sperm is disclosed. The method comprises the steps of (a) preparing an animal for semen collection; (b) collecting semen from the animal; (c) treating the collected semen with a storage buffer solution to substantially inhibit sperm motility; (d) storing the semen for a length of time and at a certain temperature; and (e) reactivating the sperm to normal motility by mixing the inhibited sperm with an activation buffer.
Claims18
A method for extending the viability of sperm which has the capacity to become motile comprising the steps of:
- (a) preparing an animal for semen collection by washing the animal with a preparation solution;
- (b) collecting semen from said animal;
- (c) treating the collected semen with a storage buffer solution to inhibit motility by at least 90 percent;
- (d) storing the semen for a length of time and at a certain temperature in said storage buffer solution;
- and
- (e) reactivating the sperm to normal motility by mixing the inhibited sperm with an activation buffer.
A method for extending the viability of sperm which has the capacity to become motile comprising the steps of:
- (a) preparing an animal for semen collection;
- (b) collecting semen from said animal;
- (c) treating the collected semen with a storage buffer solution comprising 2-[N-morpholino]-ethanesulfonic acid to inhibit motility by at least 90 percent;
- (d) storing the semen for a length of time and at a certain temperature in said storage buffer solution;
- and
- (e) reactivating the sperm to normal motility by mixing the inhibited sperm with an activation buffer.
A composition for preserving and extending the viability of immotile sperm having a pH of about 6.0, comprising about:
- (a) 50 mM potassium chloride;
- (b) 5 mM 2-[N-morpholino]ethanesulfonic acid;
- (c) 5 mM 3-[tris(hydroxymethyl)methylamino]-1 -propanesulfonic acid;
- (d) 425 mM sodium chloride;
- (e) 27 mM magnesium chloride;
- (f) 29 mM magnesium sulfate;
- (g) 10 mM calcium chloride;
- and
- (h) 2.4 mM sodium bicarbonate.
Dependencies are inferred from the claim text.